中国组织工程研究2018，Vol.22Issue(17)：2699-2704,6.DOI:10.3969/j.issn.2095-4344.0514
关节软骨损伤大鼠关节腔内移植骨髓间充质干细胞的迁移
Intra-articular migration of transplanted bone marrow mesenchymal stem cells in rats with articular cartilage injury
摘要
Abstract
BACKGROUND: The application of mesenchymal stem cells (MSCs) in the treatment of cartilage damage has become a hot spot of research. Further studies on the distribution of MSCs in the body after injection and on the underlying mechanism of action are needed. OBJECTIVE: To observe the migration of bone marrow mesenchymal stem cells (BMSCs) after injection into the region of osteochondral defect. METHODS: Thirty Sprague-Dawley rats were randomized into two groups (n=15 per group). In the control group, the femoral tochlear was exposed but an osteochondral defect was not made; and after the suture, PKH26-labeled BMSCs were directly injected into the articular cavity of rats. In the experimental group, a cartilage defect of 1 mm in diameter and 1 mm in depth was made in the rat femoral trochlea, and 5×106PKH26-labeled BMSCs were injected into the defect after operation. At 1, 3 and 7 days after injection, the femoral condyle was taken to make frozen sections followed by DAPI staining. The distribution of BMSCs was observed under laser scanning confocal microscope. RESULTS AND CONCLUSION: In the control group, PKH26-labeled BMSCs were not transferred to the subchondral bone. In the experimental group, BMSCs were detected in the subchondral bone area at 1, 3 days after injection of PKH26-BMSCs in the bone cartilage defect area, and the BMSCs were also found in the bone marrow cavity at 7 days after injection. In conclusion, BMSCs in the articular cavity cannot migrate into the subchondral bone and bone marrow cavity unless the cartilage of the femoral condyle is damaged.关键词
骨髓间充质干细胞/迁移/骨软骨缺损/PKH26/干细胞/国家自然科学基金分类
医药卫生引用本文复制引用
孙百川,王玉,张增增,周成福,彭江,王守锋,刘雪剑,张凯红,陈鹏,黄绍代,鲁长风,王冲,余文..关节软骨损伤大鼠关节腔内移植骨髓间充质干细胞的迁移[J].中国组织工程研究,2018,22(17):2699-2704,6.基金项目
国家重点研发计划(2016YFC1102104-1) （2016YFC1102104-1）
国家自然科学基金(81572148) （81572148）
北京市科技专项(Z161100005016059) （Z161100005016059）
黑龙江省教育厅科学技术研究项目(12531717) National Key Research and Development Program of China, No. 2016YFC1102104-1 （12531717）
National Natural Science Foundation of China, No. 81572148 （）
Beijing Municipal Science and Technology Project, No. Z161100005016059 （）
Science and Technology Research Project of Heilongjiang Provincial Education Department, No. 12531717 （）
