新疆农业科学2018，Vol.55Issue(4)：763-773,11.DOI:10.6048/j.issn.1001-4330.2018.04.021
中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响
Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-LβⅡ Genotype of Laying Hens
摘要
Abstract
[Objective] This experiment aims to study the effects of intramuscular injection of compound polysaccharides on immune organs, blood routine indexes and the GM-CSF, G-CSF mass fraction in differ-ent MHC B-Lβ II genotype of laying hens.[Method]500 chickens were divided into three groups according to different genotypes by PCR-SSCP method. The genotypes were divided into high, medium and low dose Chinese herbal compound polysaccharide group and blank control group. Each group of the testing chickens were intramuscularly injected with 50 mg / mL, 25 mg / mL, 12. 5 mg / mL Chinese herbal compound poly-saccharide and saline, 0. 2 mL / day for 7 days. At the age of 21, 35 and 49 days, 5 chickens were randomly selected from each group to measure the thymus, spleen and bursa of Fabricius viscera index, and blood sam-ples were collected to measure the blood routine indexes and the changes of granulocyte colony stimulating fac-tor G-CSF and granulocyte macrophage colony stimulating factor GM-CSF.[Result]The high, medium and low dose polysaccharides had certain effects on immune organs, blood routine indexes and GM-CSF in differ-ent genotype chickens, but the effects of different dosage polysaccharides on the same index of different geno-types were different. Among them, the high and medium doses of traditional Chinese medicine compound poly-saccharide could significantly improve the organ index of thymus, spleen and bursa of fabricius in AA genotype chickens, and low dose of traditional Chinese medicine compound polysaccharide could significantly improve the total number of white blood cells and lymphocytes. In the BB genotype chickens, the high and medium dose of traditional Chinese medicine compound polysaccharide could significantly improve the thymus and spleen index, but had no effect on blood routine indexes. In the BC genotype chickens, high dose of tradition-al Chinese medicine compound polysaccharide could significantly improve the thymus index, white blood cell count and lymphocyte count, and the medium dose of traditional Chinese medicine compound polysaccharide could significantly improve the total white blood cell count and lymphocyte count.[Conclusion]Different do-ses of traditional Chinese medicine compound polysaccharide can improve the immune organ index, some blood routine index and GM-CSF mass concentration of hens in different genotype groups, and in general, the immune effect of Chinese herbal compound polysaccharide with high and medium doses is better.关键词
中药复方多糖/MHC B-LβⅡ基因型/蛋鸡/免疫器官指数/血常规Key words
Chinese medicine compound polysaccharide/MHC B-LβⅡ genotype/laying hens/immune organs/blood routine indexes分类
农业科技引用本文复制引用
刘晓婷,罗燕,陈洁,连科讯,刘钢,马昭,朱晓庆,谷新利..中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响[J].新疆农业科学,2018,55(4):763-773,11.基金项目
国家自然科学基金项目"一定纯度中药复方多糖对不同 MHC B - LβⅡ 基因型鸡免疫调节作用及其作用机制研究"(31460673)The National Natural Science Foundation of China " Study on immunomodulatory effect of polysaccharides with certain purity on chicken with different MHC B-LβⅡ genotypes and its mechanism"(31460673) （31460673）
