畜牧兽医学报2018，Vol.49Issue(6)：1108-1115,8.DOI:10.11843/j.issn.0366-6964.2018.06.002
杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联
Tissue Expression Profiles of HLCS Gene and Its Association with Residual Feed Intake in Duroc
摘要
Abstract
This experiment was conducted to explore the tissue expression profile of HLCS,the polymorphisms in its coding region and the association of different genotypes with residual feed intake in Duroc.Real-time PCR was used to detect HLCS mRNA expression in 8 tissues of Duroc pigs.The polymorphic sites of HLCS were determined in Duroc with high and low RFI lines by PCR and sequencing.Genotypes were detected in Duroc,and their associations with residual feed intake trait were analyzed.Real-time PCR showed that HLCS had the highest expression in back-fat,followed by liver,and it had the lowest expression in heart and muscle.Five SNPs were de-tected in the coding region of HLCS gene.Two of them,c.1408G＞ C and c.1976A ＞ G were nonsynonymous mutations.The site c.1976A ＞ G was significantly associated with RFI(P＜0.05),and the individuals with GG genotype had lower RFI than individuals with AA genotype.These results indicate that HLCS may play an important role in porcine RFI,and c.1976A＞ G can serve as a useful genetic marker for reducing RFI in swine.This study is valuable to further explore the molecular mechanism of HLCS affecting residual feed intake.关键词
HLCS基因/RFI/SNP/猪/组织表达Key words
HLCS gene/RFI/SNP/swine/tissue expression分类
农业科技引用本文复制引用
张跃博,张龙超,蒲蕾,张金山,颜华,王立刚,侯欣华,刘欣,高红梅,王立贤..杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联[J].畜牧兽医学报,2018,49(6):1108-1115,8.基金项目
中国农业科学院科技创新工程(ASTIP-IAS02) （ASTIP-IAS02）
国家生猪产业技术体系(CARS-36) （CARS-36）
中国农业科学院北京畜牧兽医研究所中央级公益性科研院所基本科研业务费专项资金项目(2017ywf-zd-12 （）
2017ywf-zd-14 （）
2017ywf-zd-22) （）
天津市科技计划项目(15ZXZYNC00100) （15ZXZYNC00100）
天津生猪产业技术体系创新团队(ITTPRS2017006) （ITTPRS2017006）
