畜牧兽医学报2018，Vol.49Issue(6)：1116-1123,8.DOI:10.11843/j.issn.0366-6964.2018.06.003
坝上长尾鸡MITF基因核心启动子鉴定与分析
Identification and Analysis of the Core Promoter of MITF Gene in Bashang Long-tail Chicken
摘要
Abstract
The research was designed to study the activity and thestructure of MITF promoterand to provide clues for studying this gene's regulation mechanism in hair follicle.Dual-luciferase ex-pression vectors were constructed and transfected to DF1 cells with lip2000.The dual-luciferase detection kit was used to measure the relative luciferase activity.The 6 expression vectors with different promoter regions of MITF gene and 4 mutant vectors of the core promoter region were constructed in Bashang Long-tail chicken.The core promoter region from －660 bp to+200 bp was identified in chicken MITF gene.The function of －579 bp,－505 bp,－274 bp,－220 bp,－203-－202 bp,－98 bp,－46 bp,－14 bp,+1 bp and+28 bp sites in regulating promoter ac-tivity was critical.The similarities of transcription factor binding sites including HOX family(HOXA3,HOXD8,HOXD9,HOXD10 and HOX11),NF-1,DBP,TFIID and TFIIB to MITF were changed predicted by software.关键词
坝上长尾鸡/MITF基因/启动子/转录因子Key words
Bashang Long-tail chicken/MITF gene/promoter/transcription factor分类
农业科技引用本文复制引用
刘小辉,彭永东,周荣艳,张传生,李祥龙..坝上长尾鸡MITF基因核心启动子鉴定与分析[J].畜牧兽医学报,2018,49(6):1116-1123,8.基金项目
河北省蛋鸡体系产业技术创新团队(HBCT2013090206) （HBCT2013090206）
河北省科技计划项目(15226302D) （15226302D）
