畜牧兽医学报2018，Vol.49Issue(6)：1134-1144,11.DOI:10.11843/j.issn.0366-6964.2018.06.005
牛胎儿骨骼肌来源成肌细胞分化相关microRNA的筛查与鉴定
Identification of microRNAs Involved in Myogenic Differentiation of Bovine Fetal Skeletal Muscle Derived Myoblasts
摘要
Abstract
This study aimed to identify differentially expressed miRNAs in myogenic differentiation of bovine fetal skeletal muscle derived myoblasts,which would provide important insight for understanding the mechanism of myoblast differentiation at bovine fetal stage.Myoblasts were isolated and cultured from skeletal muscle of 3 bovine fetal on 4 months old,and then induced myogenic differentiation in vitro.miRNAs of myoblast at the initial and terminal stage of myoblast differentiation were sequenced using high throughput sequencing technology.The sequencing data was analyzed to identify the differentially expressed miRNAs.Differentially expressed miRNAs were randomly selected to validate the sequencing accuracy using quantitative RT-PCR.The re-sults revealed 619 miRNAs in 6 libraries,and 150 miRNAs were differentially expressed.Among them,several miRNAs were related to muscle development such as bta-miR-199a-5p.The target genes of differentially expressed miRNAs were predicted and enriched to pathways related to the muscle development such as MAPK pathway.The expression of 8 randomly selected miRNAs were quantified by real-time quantitative PCR for further validation,and their results were con-sistent with those of sequencing analyses.The study identified 150 differentially expressed miRNAs in the differentiation of bovine fetal muscle derived myoblasts.Our findings will provide valuable in-sights for further exploring the regulation roles of miRNAs in the development of bovine fetal skeletal muscle.关键词
牛/胎儿骨骼肌/miRNA/成肌细胞分化Key words
bovine/fetal skeletal muscle/miRNA/myoblast differentiation分类
农业科技引用本文复制引用
邢义珅,胡鑫,任玲,王亚慧,徐凌洋,李俊雅,张路培..牛胎儿骨骼肌来源成肌细胞分化相关microRNA的筛查与鉴定[J].畜牧兽医学报,2018,49(6):1134-1144,11.基金项目
国家自然科学基金(31672384) （31672384）
中国农业科学院科技创新工程(ASTIP-IAS03) （ASTIP-IAS03）
