畜牧兽医学报2018，Vol.49Issue(6)：1145-1153,9.DOI:10.11843/j.issn.0366-6964.2018.06.006
可控表达pGH基因转基因小鼠模型的建立与分析
Establishment and Analysis of Model of Transgenic Mice Sharing Inducible Expression of pGH
摘要
Abstract
This study aimed to construct an integrated and controllable transgenic mice model with pGH gene expression,which could reduce the side effects caused by premature expression of GH through controlling the time and quantity of GH gene expression,it would become a meaningful research to improve feed conversion of livestock.The transgenic mice were successfully obtained by prokaryotic microinjection method using a vector of Tet-on regulatory expression system,which was constructed successfully and verified through transfected PK15 cell line.Based on Southern blot results,positive Founders were bred for getting offspring.When we got the trans-genic offspring,the induction experiment was carried out with the induction substrate doxycyc-line(DOX)supplementation in the drinking water of the transgenic mice at 3-10 weeks old.RT-qPCR technology was used to detect the level of exogenous genes expression.The radioactive im-munoassay method was used to detect porcine growth hormone content in serum of mice.Body weight was weighed to evaluate the effects of pGH gene on phenotype of transgenic mice.The results showed that the body weight of induced transgenic mice was higher than that of non-induced transgenic mice and non transgenic mice in the same litter(P＜0.05);The level of pGH in serum of transgenic mice was significant higher than that of mice in control(P＜0.05),which indicated that exogenous GH could effectively express and regulate the growth of mice body.The establishment of model of pGH transgenic mice contributed to explore the effect of GH gene on body development and the molecular mechanism and signal pathway of growth hormone secre-tion.It would promote the application of GH in livestock production ;it also provided a theoreti-cal foundation and technical support for the preparation of safe and efficient transgenic livestock with GH gene.关键词
四环素可控表达系统/pGH基因/转基因小鼠Key words
Tet-on regulatory expression system/pGH gene/transgenic mice分类
农业科技引用本文复制引用
张建斌,刘颖,白立景,牟玉莲,韩俊文..可控表达pGH基因转基因小鼠模型的建立与分析[J].畜牧兽医学报,2018,49(6):1145-1153,9.基金项目
国家转基因动物新品种培育重大专项(2014zx08006005-005) （2014zx08006005-005）
中国农业科学院科技创新工程(ASTIP-IAS05) （ASTIP-IAS05）
国家自然科学基金(31501933) （31501933）
