畜牧兽医学报2018，Vol.49Issue(6)：1154-1162,9.DOI:10.11843/j.issn.0366-6964.2018.06.007
环境高温诱导巴马香猪睾丸细胞凋亡并激活Nrf2/HO-1抗氧化信号通路
Ambient High Temperature Induced Cellular Apoptosis and Nrf2/HO-1 Signal Pathway in Testes of Bama Miniature Pigs
摘要
Abstract
The objective of this study was to determine the heat sensitivity index for Bama minia-ture pigs by investigating the effect of ambient high temperature on scrotal temperature distribu-tions and testicular protein expressions related to the antioxidative capacity and cellular apoptosis in the pigs.Twelve 7-month-old mature Bama miniature pigs were selected and randomly allotted to a thermal neutral(TN)group and a heat-treated(HT)group.The HT group was exposed to high ambient temperature,which was changed from 25 to 40 ℃ and back to 25 ℃ during each 24 h period for 8 d.At the end of the experiment,testes were collected and measured for the protein expression of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase 1(HO-1)and cleaved caspase 3(c-caspase 3)using immunohistochemical staining.Results showed that,the HT group pigs presented a significant increase in scrotal surface temperature(P＜ 0.05),which was 3.7 ℃ higher than the TN group pigs.In the HT group,the testicular protein expres-sion of Nrf2 and HO-1 increased significantly in both the testicular interstitial cells and the germ line cells of seminiferous epithelium(P＜ 0.05).Moreover,prominent nuclear accumulation of Nrf2 protein was observed in the testicular interstitial cells.In the HT group,there was an in-creased percentage of the c-caspase 3-positive nucleuses in the testicular interstitial cells and germ line cells of seminiferous epithelium(P＜ 0.05),respectively.The percentage of the c-caspase 3-positive nucleuses in the testicular interstitial cells increased more sharply than that in the germ line cells of seminiferous epithelium.In conclusion,the capacity of scrotal thermal regulation was overw helmed in the 40 ℃ ambient temperature,which increased the testicular temperature and caused the testicular cell apoptosis.High ambient temperature also activated Nrf2 signal pathway and increased HO-1 expression,indicating that the testicular Nrf2/HO-1 signal pathway might be a potential target for new strategies to protect semen quality from high ambient temperature-in-duced damage in boars.关键词
环境高温/Nrf2/HO-1/睾丸/巴马香猪Key words
ambient high temperature/Nrf2/HO-1/testis/Bama miniature pig分类
农业科技引用本文复制引用
李延森,李照见,贾潇潇,李春梅..环境高温诱导巴马香猪睾丸细胞凋亡并激活Nrf2/HO-1抗氧化信号通路[J].畜牧兽医学报,2018,49(6):1154-1162,9.基金项目
国家自然科学基金(31402116) （31402116）
中央高校基本科研业务费专项资金项目(KJQN201523) （KJQN201523）
国家自然科学基金(31272485) （31272485）
