畜牧兽医学报2018，Vol.49Issue(6)：1169-1177,9.DOI:10.11843/j.issn.0366-6964.2018.06.009
发酵玉米-豆粕型全价饲料对生长猪生长性能、粪便臭味物质和菌群区系的影响
Effects of Fermented Corn-Soybean Complete Feed on the Growth Performance , Odor Concentration and Bacterial Community in Faeces of Growing Pigs
摘要
Abstract
This study was conducted to evaluate the effects of fermented complete feed(FCF)on the grow th performance,odor concentration and bacterial community in faeces of growing pigs,and evaluate the application effect of FCF in pigs.Complete feed was anaerobic fermented with Bacillus subtilis and Lactobacillus brevis for 3 days.90 pigs with the about initial weight 22 kg were randomly allotted into 3 treatment groups(antibiotic-free feed group,antibiotic feed group and FCF group)of 30 each.At the end of the experiment(body weight of pigs was up to about 40 kg),the growth performance of pigs was measured and analyzed.The fresh stool sample was collected from each group to determine the odor concentration by gas chromatography.Addition-ally,the bacterial community was assayed by using the high-throughout DNA sequencing.The results showed that :1)Fermentation of complete feed significantly increased the fat content in feed(P＜0.01)and decreased the calcium content(P＜0.01),total phosphorus content(P＜0.05)and pH(P＜0.01).The number of lactic acid bacteria in the FCF was up to 9.67 lg cfu·g－1and lactic acid content was up to 421.67 mmol·kg －1.2)Data from the animal experiment indicated that FCF feeding raised the ADFI and ADG of pigs(P＜0.05),and reduced the F/G and mortality(P＜0.05)compared with the antibiotic-free group.The ADFI of FCF-fed pigs was higher than that of anti-biotic-fed pigs(P＜0.05).3)In addition,the indole content in pig faeces was decreased(P＜0.05)by the CFC feeding.The skatole and VFA contents showed a similar decreased tendency,however,the p-cresol content showed a rising tendency in FCF group.4)Importantly,the rich-ness and diversity of faeces bacterial community were improved by the CFC feeding.The relative abundances of phyla including Tenericutes and Saccharibacteria,genera including Clostridium,Turicibacter,Mollicutes,Anaerostipes,Acetitomaculum increased ; while the relative abun-dances of genera including Lactobacillus,Streptococcus,Rikenellaceae,Megasphaera,Oscil-lospira,Mitsuokella,Prevotella reduced.In conclusion,FCF could improve the growth per-formance of pigs and the content of p-cresol in faeces,while reduce the content of indole,skatole and VFA in faeces and change the bacterial community in faeces.关键词
发酵饲料/抗生素/生长性能/臭味物质/菌群区系Key words
fermented feed/antibiotics/growth performance/odor concentration/bacterial com-munity分类
农业科技引用本文复制引用
刘志云,钟晓霞,谢跃伟,肖融,江山,黄健,黄金秀,周晓容..发酵玉米-豆粕型全价饲料对生长猪生长性能、粪便臭味物质和菌群区系的影响[J].畜牧兽医学报,2018,49(6):1169-1177,9.基金项目
重庆市基本科研业务费专项(16416) （16416）
农业部养猪科学重点实验室开放课题 （）
重庆市农发资金项目(15401) （15401）
现代农业产业技术体系建设专项资金(CARS-36) （CARS-36）
