畜牧兽医学报2018，Vol.49Issue(6)：1194-1203,10.DOI:10.11843/j.issn.0366-6964.2018.06.011
N-氨甲酰谷氨酸对育成期水貂生长性能、营养物质消化率、氮代谢及血清生化指标的影响
Effects of Dietary N-carbamoylglutamate on Growth Performance ,Nutrient Digestibility ,Nitrogen Metabolism and Serum Biochemical Indexes of Growing Mink
摘要
Abstract
This study was conducted to study the effects of different dietary N-carbamoylglutamate(NCG)levels on growth performance,nutrient digestibility,nitrogen metabolism and serum bio-chemical indexes of growing minks.One hundred and fifty healthy weaned Black minks(Mustela vison)with similar body weight were selected and randomly divided into 5 groups with 30 repli-cates per group and one mink per replicate.These minks were fed with NCG supplementation lev-els of 0％(group A),0.02％(group B),0.06％(group C),0.10％(group D)and 0.14％(group E),respectively in the experimental diets,NCG was added from June 8thto September 15th.The pre-trial lasted for 7 days and the trial for 92 days.From September 12th,10 minks(5 male and 5 female)were selected from each group for digestion and metabolism test for 3 days,muck method determination of feed and feces were conducted to measure the contents of dry matter(DM),crude protein(CP)and crude fat(EE)and then to calculate the apparent digestibility and nitrogen metabolism.On the morning of September 15th,6 minks(3 male and 3 female)were selected from each group.Blood was collected and the contents of arginine(Arg),nitric oxide(NO),growth hormone(GH),total protein(TP),alanine aminotransferase(ALT),aspartate aminotransferase(AST),immunoglobulin A(IgA),immunoglobulin G(IgG),immu-noglobulin M(IgM),complement 3(C3),complement 4(C4)in serum were determined by ELISA.The results showed that :1)The final weight and average daily gains of female minks fed with NCG were all higher than the control group,that of female minks in group B were the high-est,but the differences were not significant between different groups(P＞0.05).These indica-tors of male minks in group B was significantly higher than group A,C and E(P＜0.05).The feed/gain of female minks in group B was the lowest,but the differences were not significant between different groups(P＞0.05),the feed/gain of male minks in group B was significantly lower than A and E groups(P＜ 0.05).2)The digestibility of dry matter,crude protein and crude fat of minks fed with NCG were all higher than the control group,but the differences were not significant between different groups(P＞0.05).3)There were no significant differences in nitrogen deposition,net protein utilizable rate and biological value of protein between different groups(P＞0.05),but compared with group A,the nitrogen deposition of D group and the net protein utilizable rate,biological value of protein of B group were higher.4)Compared with group A,the levels of arginine,nitric oxide,grow th hormone,total protein,IgA,IgG,IgM,C3 and C4 in serum of minks fed with NCG extremely significantly increased(P＜0.01),and group B was all the best,the alanine aminotransferase and aspartate aminotransferase extremely signifi-cantly decreased(P＜0.01).The results indicated that basal diet supplemented with 0.02％NCG could help the growing minks to gain the average daily weight,reduce the feed/gain,improve the nitrogen deposition,net protein utilizable rate and biological value of protein,and increase the levels of arginine,grow th hormone,total protein and immunoglobulin in serum.关键词
N-氨甲酰谷氨酸/育成期水貂/生长性能/营养物质消化率/氮代谢/血清生化指标Key words
N-carbamoylglutamate/growing mink/growth performance/nutrient digestibility/nitrogen metabolism/serum biochemical index分类
农业科技引用本文复制引用
董晓静,崔凯,马泽芳,李建栋,李迎梅..N-氨甲酰谷氨酸对育成期水貂生长性能、营养物质消化率、氮代谢及血清生化指标的影响[J].畜牧兽医学报,2018,49(6):1194-1203,10.基金项目
山东省特种经济动物创新团队项目(SDAIT-21-01) （SDAIT-21-01）
山东省农业重大应用技术创新项目(鲁财农指[2015]16) （鲁财农指[2015]16）
青岛农业大学高层次人才启动基金项目(630728) （630728）
