畜牧兽医学报2018，Vol.49Issue(6)：1204-1211,8.DOI:10.11843/j.issn.0366-6964.2018.06.012
TPL2促进口蹄疫病毒在BHK-21细胞复制
TPL2 Enhances the Replication of Foot-and-mouth Disease Virus in BHK-21 Cells
摘要
Abstract
This experiment was conducted to study the effects of tumor progression locus 2(TPL2)on replication of foot-and-mouth disease virus(FMDV)in baby hamster kidney(BHK-21)cells.BHK-21 cells were infected by FMDV,the mRNA level of TPL2 was detected by qRT-PCR.TPL2 eukaryotic expression plasmid and RNAi sequences were designed and synthesized based on TPL2 sequences published in GenBank(NM007746.2).The effects of TPL2 on FMDV replica-tion in BHK-21 cells were evaluated by qRT-PCR and Western blotting.The results indicated that the transcriptional level of TPL2 was significantly up-regulated during FMDV infection ;Overexpression of TPL2 facilitated the replication of FMDV and downregulation of endogenous TPL2 inhibited FMDV replication.These results revealed that TPL2 promoted the proliferation of FMDV in BHK-21 cells.This research further expanded our understanding of the relationship between FMDV and the innate immune mechanism and had accumulated scientific data for the rel-evant research of TPL2.关键词
TPL2/口蹄疫病毒/FMDV/BHK-21细胞/天然免疫/复制Key words
TPL2/foot-and-mouth disease virus/FMDV/BHK-21 cell/innate immune/replication分类
农业科技引用本文复制引用
魏南南,徐守兴,史喜娟,卢炳州,张克山,郑海学,刘湘涛..TPL2促进口蹄疫病毒在BHK-21细胞复制[J].畜牧兽医学报,2018,49(6):1204-1211,8.基金项目
国家自然科学基金(NSFC-31572542) （NSFC-31572542）
国家科技支撑计划(2015BAD12B04) （2015BAD12B04）
