畜牧兽医学报2018，Vol.49Issue(6)：1265-1273,9.DOI:10.11843/j.issn.0366-6964.2018.06.019
广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析
Epidemiological Investigation and Genetic Diversity Analysis of Porcine Circovirus Type 3 in Slaughter Pigs of a City in Guangxi
摘要
Abstract
In order to understand the epidemic status and phylogenetic relationship of porcine circo-virus type 3 in healthy slaughter pigs,181 lymph nodes collected from slaughterhouse in Guangxi were detected for PCV3 pathogens.PCV3 infection in healthy slaughter pigs was detected by PCR method.The positive rate of PCV3 was 24.31％,which suggest that the infection of PCV3 in healthy pigs is more severe.Six PCV3 complete genome sequences were amplified with PCR in the positive samples.Compared with PCV3 complete genome in GenBank and the results showed the nucleotide homology of PCV3 sequences was 98.6％-99.9％and genes of PCV3 were very conservative and similar.Genetic evolution analysis of PCV3 ORF2 gene showed that PCV3 strains could be divided into two major types.Analyses of the phylogenetic relationship of PCVs Cap and suggesting cross protection between PCV2 and PCV3 is impossible.关键词
猪圆环病毒3型/全基因/序列分析/遗传演化分析Key words
porcine circovirus type 3/complete genenome/sequence analysis/phylogenetic analysis分类
农业科技引用本文复制引用
季程远,欧阳康,韦祖樟,黄伟坚,王蔚怡,冯选榕,姚静,黄欣,冯园青,覃一峰,方庆励,陈樱..广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析[J].畜牧兽医学报,2018,49(6):1265-1273,9.基金项目
广西科技厅重点研发项目(桂科 AB16380135) （桂科 AB16380135）
广西创新驱动发展专项资金项目(桂科 AA17204057-1) （桂科 AA17204057-1）
