畜牧兽医学报2018，Vol.49Issue(6)：1274-1281,8.DOI:10.11843/j.issn.0366-6964.2018.06.020
猪Beclin1基因调控伪狂犬病病毒诱导细胞自噬和凋亡的机制分析
The Mechanism of Porcine Beclin1 Gene on Autophagy and Apoptosis
摘要
Abstract
To study the relationship of autophagy and apoptosis after PK-15 cells were infected by pseudorabies virus(PRV),porcine host anti-apoptotic family proteins(Bcl-2 and Bcl-xL)inter-acted with Beclin1 were further analyzed.Western blotting(WB)was taken to measure the ex-pression of LC3 and P62 proteins ;and the level of apoptosis in PK-15 cells infected by PRV was investigated by Flow cytometry.Finally,Co-immunoprecipitation(Co-IP)was used to investi-gate the interaction between Beclin1 and Bcl-2/Bcl-xL.The expression of LC3-Ⅱ significantly in-creased,while that of P62 and p-AKT(S473)significantly decreased.In addition,the results of Flow cytometry showed that apoptosis was induced by PRV.Meanwhile,cleaved Caspase-9/8 significantly increased compared to control group detected by WB.Moreover,Co-IP results showed that the infection of PRV prevented the association between Beclin 1/Bcl-2 and Beclin1/Bcl-xL.PRV induces autophagy which may depend on PI3K-I/AKT and apoptosis which simulta-neously active Caspase-9 and Caspase-8,respectively.Autophagy might be induced through the interactions of Beclin1/Bcl-2 or Beclin1/Bcl-xL.关键词
PRV/细胞自噬/细胞凋亡/Beclin1Key words
pseudorabies virus/autophagy/apoptosis/Beclin1分类
农业科技引用本文复制引用
胡林,南良康,张敏,许苏童,杨了寒,李帅峰,王凯,刘正飞,张淑君..猪Beclin1基因调控伪狂犬病病毒诱导细胞自噬和凋亡的机制分析[J].畜牧兽医学报,2018,49(6):1274-1281,8.基金项目
国家自然科学基金(31572367 （）
31272427) （）
