畜牧兽医学报2018，Vol.49Issue(6)：1299-1306,8.DOI:10.11843/j.issn.0366-6964.2018.06.023
伊犁马ACTN3基因外显子多态性研究
Analysis on ACTN3 Gene Exon Polymorphism of Yili Horse
摘要
Abstract
The aims of this study were to identify genetic variants and construct haplotypes of polymorphic sites of ACTN3 gene exon in Yili horse,and determine different haplotypes effects on the mRNA expression level and protein secondary stucture of ACTN3,which would provide a theoretical basis for further researching the influence of the gene expression on athletic perform-ance.Direct sequencing was taken to detect the polymorphic sites of of ACTN3 of 38 Yili horse and analyze its genotypes.The haplotypes and bioinformation were analyzed by haploview 4.2 and online software(RNAfold and sopm),respectively.A total of 8 SNPs were identified from the ACTN3 gene exon,only T9059G was the missense mutant,and the other 7 were synonymous mutants.Based on the 8 SNPs,linkage analysis showed that the strong linkage between A6206G and A11517G,and the different linkage relationship among the other SNPs.There was 14 haplo-types by the 8 SNPs,H1(GGATCCCG)was the dominant haplotype,and the different haplo-types could cause the change of mRNA secondary structure and minimum free energy of ACTN3 gene.The missense mutation of T9059G changed the secondary structure of ACTN3 protein.The result indicate that the T9059G is one of the improtant functional sites affecting athletic per-formance,which affect the secondary structure of ACTN3 protein.关键词
伊犁马/ACTN3基因/遗传多态性/生物信息学Key words
Yili horse/ACTN3 gene/genetic polymorphism/bioinformatics分类
农业科技引用本文复制引用
王建文,李林玲,王镜力,孟军,曾亚琦,姚新奎,辛雅莉..伊犁马ACTN3基因外显子多态性研究[J].畜牧兽医学报,2018,49(6):1299-1306,8.基金项目
新疆维吾尔自治区重大科技专项(2017A01002-1) （2017A01002-1）
新疆维吾尔自治区天山创新团队计划 （）
中国博士后基金委、新疆维吾尔自治区大学生创新创业训练计划项目(201710758086) （201710758086）
