畜牧兽医学报2018，Vol.49Issue(6)：1307-1313,7.DOI:10.11843/j.issn.0366-6964.2018.06.024
ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位
Expression and Localization of ANXA2 and ERp29 in Mammary Gland Tissue of Normal and Clinical Mastitis Sheep(Ovis aries)
摘要
Abstract
The study aimed to investigate the expression and localization of annexin2(ANXA2)and endoplasmic reticulum protein 29(ERp29)genes in mammary gland tissue of normal and clinical mastitis sheep(Ovis aries).In this study,mammary gland tissues of 3 normal and 3 clini-cal mastitis sheep(Ovis aries)were sampled.qRT-PCR,Western blot(WB)and immunohisto-chemistry(IHC)were used to detect the expression and distribution of ANXA2 and ERp29 mR-NA and proteins in mammary gland tissue of normal and clinical mastitis sheep.The results showed that the expression of ANXA2 mRNA in the mammary gland tissue of clinical mastitis sheep was significantly higher than that of normal group(P＜ 0.01),while the expression of ANXA2 protein was significantly lower than that of normal group(P＜0.01).The expression of ERp29 mRNA and protein in the mammary gland tissue of clinical mastitis sheep were signifi-cantly higher than that of normal group(P＜0.01).ANXA2 and ERp29 proteins had positive reactions in mammary gland tissues of normal and clinical mastitis sheep respectively and mainly located in mammary epithelial cells.The expression of ANXA2 and ERp29 were significantly dif-ferent in the mammary gland tissue of normal and clinical mastitis sheep,which may be involved in the regulation of the occurrence and development of sheep mastitis.关键词
绵羊/乳房炎/ANXA2/ERp29/表达/定位Key words
sheep/mastitis/ANXA2/ERp29/expression/location分类
农业科技引用本文复制引用
高建锋,李讨讨,陈灿灿,赵兴绪,马友记..ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位[J].畜牧兽医学报,2018,49(6):1307-1313,7.基金项目
甘肃省科技重大专项计划(1602NKDH020) （1602NKDH020）
甘肃农业大学校级课题(GSAU-ZL-2015-030) （GSAU-ZL-2015-030）
国家肉羊产业技术体系项目(CARS-38) （CARS-38）
