中国病理生理杂志2018，Vol.34Issue(6)：961-968,8.DOI:10.3969/j.issn.1000-4718.2018.06.001
过表达微小RNA-21对c-Kit+心脏干细胞增殖、迁移及分化的影响
Effect of miR-21 over-expression on proliferation, migration and diffe-rentiation of c-Kit+ cardiac stem cells
摘要
Abstract
AIM:To explore the effect of microRNA (miR)-21 on proliferation, migration and differentiation abilities of c-Kit+ cardiac stem cells (CSCs). METHODS:c-Kit+ CSCs were cultured and selected by the methods of en-zyme digestion and magnetic bead separation. miR-21 mimics (50 nmol/L) and mimics negative control ( MNC) were transfected into c-Kit+CSCs with Lipofectamine?2000. The cells was divided into 3 groups:control group:c-Kit+ CSCs without any pretreatment; MNC group:the cells were transfected with MNC for 48 h; mimics group:the cells were trans-fected with miR-21 mimics for 48 h. qPCR was used to assess the expression of miR-21 in each group. CCK-8 and EdU as-says were used to determine the cell proliferation. qPCR and immunofluorescence were used to detect the differentiation in each group. Scratch assay was adopted to explore the migration ability of the cells. RESULTS:The expression of c-Kit in the c-Kit+ CSCs were 90.8%, with 0.6% of CD45 and 0.5% of CD34. A significant increase in miR-21 expression was observed when the cells were transfected with miR-21 mimics for 48 h ( P<0.05). CCK-8 and EdU assays showed that miR-21 significantly increased cell proliferation as compared with MNC group and control group (P<0.05). No difference in the expression of Nkx2.5, CD31 and α-SMA at mRNA and protein levels was observed, and no difference of the migra-tion ability in 3 groups of the c-Kit+ CSCs was found. CONCLUSION:Over-expression of miR-21 significantly promotes the proliferation of c-Kit+ CSCs, without any effect on the cell migration and differentiation.关键词
微小RNA-21/心脏干细胞/细胞增殖/细胞迁移/细胞分化Key words
MicroRNA-21/Cardiac stem cells/Cell proliferation/Cell migration/Cell differentiation分类
医药卫生引用本文复制引用
王艳,石蓓,邓文文,王冬梅,盛瑾,陈文明..过表达微小RNA-21对c-Kit+心脏干细胞增殖、迁移及分化的影响[J].中国病理生理杂志,2018,34(6):961-968,8.基金项目
国家自然科学基金资助项目(No.81760041) （No.81760041）
贵州省科技项目(黔科合LH字[2017]7107号) （黔科合LH字[2017]7107号）
