中国病理生理杂志2018，Vol.34Issue(6)：1020-1024,5.DOI:10.3969/j.issn.1000-4718.2018.06.010
AZD8055对胆管癌细胞自噬和凋亡的影响
Effects of AZD8055 on autophagy and apoptosis in cholangiocarcinoma cells
摘要
Abstract
AIM:To explore the effects of mammalian target of rapamycin (mTOR) double inhibitor AZD8055 on autophagy and apoptosis of human cholangiocarcinoma cell line HuCCT1. METHODS:The effect of AZD8055 on the viability of HuCCT1 cells was detected by MTT assay. Autophagosome was detected by acridine orange (AO) staining. Af-ter treated with AZD8055, the expression levels of apoptosis-related proteins Bcl-2, Bax and cleaved caspase-3 and auto-phagy marker proteins beclin 1, LC3 and p62 were determined by Western blot. Apoptotic rate was analyzed by flow cyto-metry with Annexin V-FITC/PI double staining. RESULTS:AZD8055 significantly inhibited the viability of HuCCT1 cells (P<0.05). AO staining showed that AZD8055 significantly increased orange granules in the cytoplasm. After treated with AZD8055, compared with the control group, the protein level of beclin 1 and the ratio of LC3-Ⅱ/LC3-Ⅰ were enhanced, while p62 was attenuated (P<0.05). The protein expression level of pro-apoptotic regulator Bax was down-regulated and anti-apoptotic regulator Bcl-2 was increased. The protein level of cleaved caspase-3 was reduced (P<0.05). The results of flow cytometry showed that AZD8055 inhibited cell apoptosis. CONCLUSION:AZD8055 inhibits the viability of cholangiocarcinoma cells, and the mechanism is closely related with autophagy induced by AZD8055.关键词
AZD8055/胆管癌/自噬/细胞凋亡Key words
AZD8055/Cholangiocarcinoma/Autophagy/Apoptosis分类
医药卫生引用本文复制引用
刘特思,闫文帝,王雪,吕游,朴英实,林贞花,任香善..AZD8055对胆管癌细胞自噬和凋亡的影响[J].中国病理生理杂志,2018,34(6):1020-1024,5.基金项目
国家自然科学基金资助项目(No.413010033) （No.413010033）
