中国肺癌杂志2018，Vol.21Issue(6)：431-436,6.DOI:10.3779/j.issn.1009-3419.2018.06.01
吉非替尼耐药的人肺腺癌HCC-827/GR细胞乙醛脱氢酶亚型表达分析
Expression of Acetaldehyde Dehydrogenase in Gefitinib-resistant Human Lung Ad-enocarcinoma HCC-827/GR Cells
摘要
Abstract
Background and objective Tumor recurrence and drug resistance are the main causes of death in tumor patients. The family of acetaldehyde dehydrogenase (ALDH) is closely related to the proliferation, migration, invasion and resistance of tumor cells, and different ALDH subtypes are expressed in different tumor cells. The aim of this study is to elucidate the ALDH subtype in human lung adenocarcinoma HCC-827/GR cells, which resistant to the gefitinib. Methods The human lung adenocarcinoma HCC-827 cells were used to generate the gefitinib-resistant HCC-827/GR cells; the expression of ALDH subtype in either HCC-827 or HCC-827/GR was detected by flow cy-tometry; The proliferative capacity and sensitivity to gefitinib of hcc-827/GR cells were analyzed by MTT assay before and after treatment with 100 μmol/L diethyllaminaldehyde (DEAB); Real-time quantitative PCR was used to detect the expression of ALDH subtypes at mRNA levels in hcc-827 cells and hcc-827/GR cells. Results Compared with HCC-827 cells, the positive rate of ALDH in HCC-827/GR cells increased. The proliferation ability of HCC-827/GR cells decreased after treatment with 100 μmol/L DEAB. Compared with HCC-827 cells, the expression of ALDH1A1 and ALDH1L1 mRNA was increased in hcc-827/GR cells, but the ALDH3B2 expression was decreased. Conclusion ALDH might be used as a molecular biomarker to test the gefitinib-resistant to lung adenocarcinoma cancer cells, and the ALDH1A1 may play a role in gefitinib resistance in lung cancer.关键词
人肺腺癌/HCC-827/吉非替尼/乙醛脱氢酶Key words
Human lung adenocarcinoma/HCC-827/Gefitinib/Acetaldehyde dehydrogenase引用本文复制引用
杨婷婷,古晶晶,刘婷,马海滨,马晓娜,陶金,金毅然,梁雪云..吉非替尼耐药的人肺腺癌HCC-827/GR细胞乙醛脱氢酶亚型表达分析[J].中国肺癌杂志,2018,21(6):431-436,6.基金项目
本研究受宁夏医科大学科学基金资助项目(No.XQ201332)资助 This study was supported by the grant from Projects funded by the Ningxia Medical University (to Tingting YANG)(No.XQ201332). （No.XQ201332）
