中国肺癌杂志2018，Vol.21Issue(6)：470-475,6.DOI:10.3779/j.issn.1009-3419.2018.06.08
肺腺癌长链非编码RNA启动子DNA甲基化的生物信息学分析
Bioinformatics Analysis of DNA Methylation in the Promoters of LincRNA in Lung Adenocarcinoma
张文 1李少军 1郭楠楠 1赵英男1
作者信息
- 1. 100048 北京,中国人民解放军总医院第一附属医院胸外科
- 折叠
摘要
Abstract
Background and objective Previous studies have found the lincRNAs play important roles in the oc-currence and development of lung adenocarcinoma, but their regulated mechanims are still unclear. The aim of this study is to evaluate the relationship between DNA methylation and lincRNA expression, and the effect of prognosis in lung adenocarci-noma. Methods The whole genome Illumina Methylation 450 DNA methylatiuon data and RNAseq for lung adenocarcinoma were download from TCGA. DNA methylation around lincRNA and their relationship to gene expression were analyzed.Their contribution to lung adenocarcinoma were further analyzed by comparing DNA methylation and lincRNA expression in tumor and adjacent tissues. Results The methylation level in promoter region was lower than other positions in lincRNA, and was negatively correlated with gene expression. There were 427 lincRNA genes showed differential DNA methylation in their promoter regions in tumor and adjacent tissues. Among these, 15 lincRNA genes showed differential gene expression and nega-tively correlated with DNA methylation, including FENDRR (a tumor progression and metastasis gene) whose high methyla-tion in its promoter showed poor survival in lung adenocarcinoma. Conclusion The expression of lincRNA can be regulated by DNA methylation in their promoter regions, and the level of DNA methylation is related to patient prognosis in lung adeno-carcinoma.关键词
肺腺癌/LincRNA/DNA甲基化/预后Key words
Lung adenocarcinoma/LincRNA/DNA methylation/Prognosis引用本文复制引用
张文,李少军,郭楠楠,赵英男..肺腺癌长链非编码RNA启动子DNA甲基化的生物信息学分析[J].中国肺癌杂志,2018,21(6):470-475,6.
