中国骨伤2018，Vol.31Issue(6)：550-555,6.DOI:10.3969/j.issn.1003-0034.2018.06.013
Piezo1蛋白在人退变软骨细胞应力模型中的表达特点
Expression characteristics of Piezo1 protein in stress models of human degenerative chondrocytes
摘要
Abstract
Objective:To explore the expression characteristics of new mechanosensitive ion channel Piezo1 protein in stress models of human degenerative chondrocytes. Methods:The stress stimulation model of human degenerative chondrocytes in vitro was constructed. Multi channel cell stretch stress loading system FX-4000T was used to treat chondrocytes. According to the results of pre test, the loading frequency of 0.5 Hz and the cell elongation of 20％ were loaded. According to cell processing time, it was divided into 0 h, 2 h, 12 h, 24 h and 48 h mechanical stress group. The RT-PCR and Western-blot were used to test the expression of the Piezo1, also the Laser scanning confocal microscope (LSCM) was used to test the intensity of the fluorescence of the Piezo1. Results:(1)The result of the RT-PCR showed that the expression of the Piezo1 in the 2 h group was higher than the 0 h group (F=13.917, q=0.037 1, P<0.05). The expression of the piezo1 in the 24 h group was the highest. While the expression of the piezo1 in the 48 h group was lower than the expression of the piezo1 in the 24 h group (F=13.917, q=0.049 5, P<0.05). (2)The result of the Western-blot showed that the 2 h group was higher than the 0 h group (F=19.341, q= 0.037 1, P<0.05). The expression of the 24 h had the highest expression which was higher than the 48 h group (F=19.341, q= 0.017 7, P<0.05). (3) The Piezo1 protein was extensively expressed in the cytoplasm and nucleus of the nucleus pulposus cells. And with the increase of stress processing time, the fluorescence intensity of the protein also increased. Conclusion:In human degeneration cartilage cells, the new mechanio sensitive ion channel Piezo1 protein has a trace expression. After loading periodic mechanical tensile force, the expression of Piezo1 protein increases with time dependence.关键词
Piezo1蛋白离子通道/机械牵张应力/软骨细胞Key words
Piezo1 protein ion channel/Mechanical stretch force/Chondrocytes引用本文复制引用
杨骐宁,曹杨,周勇伟,倪硕,傅蔚聪,施铁军,李晓飞..Piezo1蛋白在人退变软骨细胞应力模型中的表达特点[J].中国骨伤,2018,31(6):550-555,6.基金项目
国家自然科学基金(编号:81272056) （编号:81272056）
Provided by the National Natural Science Foundation of China(No. 81272056) （No. 81272056）
