中国康复理论与实践2018，Vol.24Issue(6)：629-633,5.DOI:10.3969/j.issn.1006-9771.2018.06.002
人参皂苷Rb通过减轻线粒体损伤对大鼠脊髓缺血再灌注损伤的保护作用
Protective Effect of Ginsenoside Rb1 on Spinal Cord Ischemic-reperfusion Injury in Rats by Alle-viating Mitochondrial Damage
摘要
Abstract
Objective To observe the effects of ginsenoside Rb1 pretreatment on spinal cord ischemia-reperfusion injury in rats, and to explore the possible mitochondrial mechanism of ginsenoside Rb1 against ischemia-reperfusion injury. Methods The spinal cord ischemia-reperfusion injury model was established. The rats were randomly divided into sham operation group (n=12), ischemia-reperfusion group (n=12) and drug group (n=12). The drug group received gin-senoside Rb1 peritoneal injection with 10 mg/kg, 20 mg/kg, 40 mg/kg and 80 mg/kg, respectively, 30 minutes be-fore modeling and once a day after modeling. After 48 hours of reperfusion, the BBB score was tested, the levels of superoxide dismutase (SOD) and malonaldehyde (MDA) in the serum and spinal cord tissue, and the cyto-chrome C oxidase (COX) activity in spinal cord tissue were detected. Results Compared with the ischemia-reperfusion group, the BBB score increased (P<0.05), the SOD level increased, and the MDA level decreased both in serum and spinal cord tissue, the activity of COX increased in the spinal cord tissue (P<0.05). All the indexes were dose-dependent, however, no difference was found between 40 mg/kg and 80 mg/kg. Conclusion Ginsenoside Rb1 can inhibit oxidative stress induced by spinal cord ischemia-reperfusion injury in rats by inhibiting mitochondrial damage. The protective effect of ginsenoside Rb1 on the spinal cord ischemia-reperfu- sion injury is dose-dependent during 10 to 40 mg/kg dose.关键词
脊髓损伤/缺血再灌注/人参皂甙Rb1/线粒体/大鼠Key words
spinal cord injury/ischemia-reperfusion/ginsenoside Rg1/mitochondria/rats分类
医药卫生引用本文复制引用
程斌,杨峰,李锋涛,林磊,薛建利,吴昊,叶劲涛..人参皂苷Rb通过减轻线粒体损伤对大鼠脊髓缺血再灌注损伤的保护作用[J].中国康复理论与实践,2018,24(6):629-633,5.基金项目
陕西省自然科学基金面上项目(No. 2014JM2_8157)Shaanxi Natural Science Foundation (General) (No. 2014JM2_8157) （No. 2014JM2_8157）
