中国康复理论与实践2018，Vol.24Issue(6)：634-640,7.DOI:10.3969/j.issn.1006-9771.2018.06.003
超短波促进巨噬细胞表型转化并抑制大鼠脊髓损伤后炎症反应的作用
Effect of Ultrashort Wave on Macrophage Polarization and Inflammatory Response after Spinal Cord Injury in Rats
摘要
Abstract
Objective To explore the effect of ultrashort wave on macrophage polarization and inflammation response after spinal cord injury in rats. Methods A total of 96 Sprague-Dawley rats were randomly divided into sham group, model group and ultrashort wave group, with 32 rats in each group. The spinal cord injury model was established by modified Allen's method in the model group and the ultrashort wave group. The ultrashort wave group received ultrashort wave since the first day after modeling. BBB score was applied to evaluate the locomotion recovery. HE staining was used to as-sess the injury area, while immunochemistry, real-time PCR and Western blotting were applied to measure the mRNA and protein expression of inducible nitric oxide synthase (iNOS), arginase-1 (Arg-1) and tumor necrosis factor-α (TNF-α). Results Compared with the model group, the BBB score increased (P<0.05), the expression of iNOS and TNF-α de-creased, and the expression of Arg-1 increased (P<0.05). Conclusion Ultrashort wave therapy may promote macrophage polarization from M1 to M2, inhibit inflammatory re-sponse and promote recovery after spinal cord injury in rats.关键词
脊髓损伤/超短波/巨噬细胞表型转化/肿瘤坏死因子-α/修复/大鼠Key words
spinal cord injury/ultrashort wave/macrophage polarization/tumor necrosis factor-α/repair/rats分类
医药卫生引用本文复制引用
高琳,冯智萍,代辰飞,张志强,张立新..超短波促进巨噬细胞表型转化并抑制大鼠脊髓损伤后炎症反应的作用[J].中国康复理论与实践,2018,24(6):634-640,7.基金项目
1.国家自然科学基金项目(No. 81101462) （No. 81101462）
2.辽宁省自然科学基金项目(No. 201602875) （No. 201602875）
3.辽宁省公益科学基金项目(No. 2016003001)National Natural Science Foundation of China (No. 81101462), Liaoning Natural Science Foundation (No. 201602875) and Liaoning Public Science Foundation (No. 2016003001) （No. 2016003001）
