中国临床药理学杂志2018，Vol.34Issue(9)：1077-1080,4.DOI:10.13699/j.cnki.1001-6821.2018.09.020
葛根素抑制软脂酸诱导的小鼠3T3-L1细胞炎性因子的表达及其机制
Inhibition effect of puerarin on the expression of inflammatory factors in 3T3-L1 cells induced by palmitic acid and its mechanism in mice
摘要
Abstract
Objective To explore the effect and mechanism of puerarin on inflammatory response in murine 3T3-L1 adipocytes.Methods Tolllike receptor 4 (TLR4) mediated inflammatory cell models were based on palmitic acid (PA) 200 μmol · L-1 stimulated 3T3-L1 adipocytes cells.And then,these cells were divided into 4 groups:control group(without PA induction in 3T3-L1 cells),model group,low and high dose experimental groups (5,10 μmol · L-1 puerarin).The mRNA expression of TLR4 was detected by Real-time PCR.The tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) were performed using enzyme-linked immuno sorbent assay.The protein expressions of inhibitor of nuclear factor kappa-B kinase β (IKKβ),nuclear factor kappa B inhibitory protein(IκB),nuclear factor kappa B-p65 (NF-κB p65) in 3T3-L1 adipocytes cells were assessed by Western blot.Results The mRNA expression of TLR4 in control group and model group were 1.20 ± 0.20,6.23 ± 0.80;the level of TNF-α in the two groups were (20.40 ± 2.0),(60.20 ± 8.0) pg · mL-1;the level of IL-6 in the two groups were (4.20 ± 0.2),(14.40 ± 0.4) pg · mL-1.Comparison between control group and model group,the diffemce on the three factors had significantly(all P ＜ 0.01).Meanwhile,after administration of puerarin,the protein expressions of IKKβ,IκB and NF-κ-p65 in model group were 2.10 ±0.4,4.20 ±0.5,5.50 ±0.2;that in experimental-H group were 1.40 ± 0.3,2.10 ± 0.4,3.60 ± 0.6.Comparison between experimental-H group and model group,the differnce on the three factors had significantly(all P ＜ 0.01).Conclusion Experimental-H group attenuates palmitate-induced inflammatory response in 3T3-L1 adipocytes,its underlying mechanism include inhibiting expression of TLR4 and downregulating activation of IKKβ,IκB,NF-κB-p65 and decreased expression of TNF-α,IL-6 levels.关键词
葛根素/软脂酸/脂肪细胞/Toll样受体4/炎性细胞因子Key words
puerarin/palmitic acid/adipocyte/Toll-like receptor 4/inflammatary cytokin分类
医药卫生引用本文复制引用
王小康,许耿瑞,吴铁松,刘江红,谢展雄..葛根素抑制软脂酸诱导的小鼠3T3-L1细胞炎性因子的表达及其机制[J].中国临床药理学杂志,2018,34(9):1077-1080,4.基金项目
广东省科技计划基金资助项目(2009B030801031) （2009B030801031）
深圳市龙华新区科技创新基金资助项目(20160523A1030149) （20160523A1030149）
