王琳琳 1冯佳莉 1刘俊芬 1郝秀轻 2左路广 3刘圣君 1刘华1
作者信息
- 1. 河北北方学院附属第一医院肾内科,河北张家口075000
- 2. 河北北方学院附属第一医院病理科,河北张家口075000
- 3. 河北北方学院附属第一医院检验科,河北张家口075000
- 折叠
摘要
Abstract
Objective To explore the protective effect of astragaloside Ⅳ on the oxidative stress injury of human glomerular mesangial cells under high glucose conditions and the mechanism.Methods The glomerular mesangial cells were dividedinto model group,control group and experimental-L/M/H groups.Model group was treated with 25 mmol · L-1 glucose,experimental-L/M/H groups were given 25,50,100 μmol · L-1 astragaloside Ⅳ on the basis of model group,control group was treated with equivalent normal saline.Each group was cultured for 48 h after inervention.The cells proliferation were detected by methyl thiazolyl tetrazolium (MTF),the reactive oxygen species (ROS) level and fluorescence intensity were detected by fluorescent probe method,the cells Akt/NF-κB signaling pathways related proteins were detected by Western blot.Results The proliferation inhibition rates of glomerular mesangial cells in experimental-L/M/H groups were (6.79 ± 0.12) ％,(17.05 ± 0.15) ％,(28.31 ± 0.23) ％,and increased with the increasing of astragaloside Ⅳ concentration(P ＜0.01).The ROS relative expressions of glomerular mesangial cells in control group,model group and experimental-L/M/H groups were 0.02 ± 0.01,0.07 ± 0.01,0.06 ± 0.02,0.04 ± 0.03,0.02 ± 0.02;the phospho-protein binases (p-Akt) protein relative expression were 0.25 ± 0.03,0.57 ± 0.02,0.46 ± 0.02,0.31 ± 0.04,0.27 ± 0.03;p-IκBαprotein relative expression were 0.44 ± 0.03,0.16 ±0.01,0.21 ±0.02,0.26 ±0.02,0.33 ±0.02.Compared with control group,the ROS relative expression,fluorescence intensity and p-Akt protein relative expression of model group increased significandy (P ＜ 0.01),the p-IκBα protein relative expression decreased significantly (P ＜0.01).Compared with model group,the ROS relative expression,fluorescence intensity and p-Akt protein relative expression of experimental groups decreased significantly with the increasing of astragaloside Ⅳ concentration (P ＜ 0.01);while the p-IκBα relative expression increased with the increasing of astragaloside Ⅳ concentration (P ＜ 0.01).Conclusion Astragaloside Ⅳ can adjust the ROS expression level of glomerular mesangial cells under high glucose conditions effectively,and inhibit the oxidative stress reaction,the action mechanism maybe related to the down-regulation of p-Akt protein and the up-regulation of p-IκBα protein of Akt/NF-κB signaling pathways,which has significant dose-dependent manner.关键词
肾小球系膜细胞/高糖环境/黄芪甲苷/氧化应激/机制Key words
glomerular mesangial cell/high glucose conditions/astragaloside Ⅳ/oxidative stress/mechanism分类
医药卫生
