中国临床药理学杂志2018，Vol.34Issue(10)：1168-1171,4.DOI:10.13699/j.cnki.1001-6821.2018.10.010
微小RNA-130b在转化生长因子β1诱导的肾小球系膜细胞纤维化中的调控作用
Regulation of miR-130b on glomerular mesangial cell fibrosis induced by transforming growth factor β1
摘要
Abstract
Objective To investigate the effect of miR-130b on the fibrosis of human mesangial cells (HMC) induced by transforming growth factor β 1 (TGF-β1).Methods In vitro HMC cells were cultured with high glucose (25 mmol · L-1) or low glucose (5.5 mmol · L-1),treated by miR-130b high expression of lentivirus or TGF-β1,respectively divided into low glucose,high glucose 24,48 h groups,10,30 ng · mL-1TGF-β1 24,48 h groups,miR-130b lentivirus group and miR-130b-NC group.Western blot and immunofluorescence technique were used to detect the expression of collagen type Ⅰ protein (col Ⅰ),and the expression of miR-130b and col Ⅰ mRNA were detected by real -time polymerase chain reaction (qRT-PCR).Results Induced by TGF-β1,the expression of miR-130b in miR-130b lentivirus group and miR-130b-NC group were 6.01 ± 1.09,1.00;col Ⅰ mRNA expression were 2.88 ± 0.64,1.00,and col Ⅰ potein expression were 2.11 ±0.22,1.00,all with significant difference (all P ＜ 0.01).Conclusion miR-130b,col Ⅰ mRNA and protein were all highly expressed in the process of mesangial cell fibrosis induced by TGF-β1,and miR-130b may be related to the induction of mesangial cell fibrosis induced by TGF-β1.关键词
人肾小球系膜细胞/纤维化/转化生长因子β1/微小RNA-130bKey words
human glomerular mesangial cell/fibrosis/transforming growth factor β1/microRNA-130b分类
医药卫生引用本文复制引用
史景霞,王艳,马瑜瑾,李世朋,王洁,姜宏卫,张鑫..微小RNA-130b在转化生长因子β1诱导的肾小球系膜细胞纤维化中的调控作用[J].中国临床药理学杂志,2018,34(10):1168-1171,4.基金项目
国家自然科学基金资助项目(U1404805) （U1404805）
河南省高等学校重点科研基金资助项目(16A320004) （16A320004）
