中国临床药理学杂志2018，Vol.34Issue(10)：1172-1174,1182,4.DOI:10.13699/j.cnki.1001-6821.2018.10.011
蟾毒灵对人结肠癌HCT116细胞有氧糖酵解的影响
Effect of Bufalin on aerobic glycolysis in colorectal cancer HCT116 cell
摘要
Abstract
Objective To investigate the effect of Bufalin on aerobic glycolysis in human colorectal cancer cell.Methods The colorectal cancer cells were divided into Bufalin groups and control group.Bufalin groups were treated with Bufalin at various concentrations(5,10,20,40nmol · L-1) for 48 h and control group was given the same dose of complete medium.The level of intracellular ATP level and cell lacate production were determined by Kit.The C-myc and energy metabolism regulator were measured by Western blot.Results Compared with the control group(1.00),4 concentrations (5,10,20,40 nmol · L-1) Bufalin were 91.69％,78.00％,68.55％,54.03％ on the inhibition of human colon cancer cell line HCT116 in ATP level rate;the 4 concentrations Bufalin were 89.04％,77.27％,59.66％,47.52％ to inhibit HCT116 cell junction formation of lactic acid in colorectal cancer cell line rate,the difference was statistically significant (P ＜ 0.05,P ＜ 0.01).Compared with the control group (1.00),the 4 concentrations Bufalin with HCT116 cells C-myc protein colon cancer cell line expression ratio were 0.95,0.84,0.73,0.68;lactate dehydrogenase A (LDH-A) expression ratio were 0.95,0.90,0.79,0.60,the difference was statistically significant (P ＜ 0.05,P ＜ 0.01).Conclusion Bufalin can inhibit the energy metabolism of colorectal cancer cell,which may be related with the down-regulation of C-myc expression.关键词
蟾毒灵/结肠癌/细胞株HCT116/有氧糖酵解Key words
Bufalin/colorectal cancer/cell line HCT116/aerobic glycolysis分类
医药卫生引用本文复制引用
袁玉霞,贺雪,李喆,唐雪瑶,张祎稀,邱艳艳,袁泽婷,殷佩浩..蟾毒灵对人结肠癌HCT116细胞有氧糖酵解的影响[J].中国临床药理学杂志,2018,34(10):1172-1174,1182,4.基金项目
国家自然科学基金面上基金资助项目(81473482) （81473482）
上海中医药大学中西医结合高原学科面上基金资助项目(16-DX-08) （16-DX-08）
上海中医药大学“远志杯”研究生科创基金资助项目 （）
