中国临床药理学杂志2018，Vol.34Issue(10)：1179-1182,4.DOI:10.13699/j.cnki.1001-6821.2018.10.013
苦参碱对宫颈癌SiHa细胞增殖的影响及其对JAK-SATA通路负调控机制的研究
Effect of Matrine on the proliferation of SiHa cells and the negative regulation of JAK-SATA pathway
摘要
Abstract
Objective To explore the effect of Matrine on the proliferation of human cervical carcinoma SiHa cells and analyze the negative regulation mechanism of JAK-SATA pathways.Methods The human cervical carcinoma SiHa cells were managed by 0.25,0.50,1.00,2.00,4.00 mg· mL-1 Matrine as 0.25,0.50,1.00,2.00,4.00 mg · mL-1 test groups,each hole 100 μL,set the control group at the same time (managed by equivalent normal saline).After 48 h intervened,the morphological changes of human cervical carcinoma SiHa cells apoptosis were observed under inverted microscope;the proliferation of human cervical carcinoma SiHa cells and the expression of JAK-SATA pathways related protein were compared by methyl thiazolyl tetrazolium (MTT) and Western blot methods.Results At 72 h after intervened,the anti-proliferative rates of human cervical carcinoma SiHa cells in 0.25,0.50,1.00,2.00,4.00 mg·mL-1 test groups were (17.11 ±3.04)％,(33.04 ±2.85)％,(48.13 ±3.54)％,(61.63 ±5.87)％,(72.90 ± 7.99) ％.The anti-proliferative rates of human cervical carcinoma SiHa cells showed a trend of gradually increased with the increasing of Matrine concentration and the extension of action time (P ＜ 0.05 or P ＜ 0.01).Western blot showed that the relative expression of JAK1 protein in control group and 0.50,1.00,2.00 mg · mL-1 test groups were 0.63 ± 0.04,0.60 ± 0.04,0.53 ± 0.04,0.27 ± 0.03;the relative expression of JAK2 protein were 0.72 ± 0.04,0.64 ± 0.04,0.57 ± 0.04,0.50 ± 0.03;the relative expression of STAT3 protein were 0.59 ± 0.03,0.54 ± 0.03,0.47 ± 0.04,0.41 ± 0.03.Compared with control group,the protein expression of JAK1,JAK2,STAT3 in test groups decreased significantly,and the protein expression of JAK1,JAK2,STAT3 decreased gradually with the increasing of Matrine concentration.Conclusion Matrine can inhibit the proliferation of human cervical carcinoma SiHa cells,and which shows dose-time dependence,the related action mechanism maybe related to the reduction of the signaling transduction activity of JAK-SATA pathway in human cervical carcinoma SiHa cells.关键词
宫颈癌/苦参碱/SiHa细胞/JAK-SATA通路/增殖Key words
cervical carcinoma/Matrine/SiHa cell/JAK-SATA pathway/proliferation分类
医药卫生引用本文复制引用
闫文明,高宇,郁志龙,张保祯,赵建国,李心红,张姝凤..苦参碱对宫颈癌SiHa细胞增殖的影响及其对JAK-SATA通路负调控机制的研究[J].中国临床药理学杂志,2018,34(10):1179-1182,4.基金项目
内蒙古自治区财政厅基金资助项目(2015cztcxy0504) （2015cztcxy0504）
