中国临床药理学杂志2018，Vol.34Issue(10)：1195-1198,4.DOI:10.13699/j.cnki.1001-6821.2018.10.017
当归对血虚便秘模型小鼠胃肠激素与酪氨酸激酶受体C-kit表达的影响
Effects of Angelica sinensis radix on gastrointestinal hormone and expresomatostatinion of tyrosine kinase receptor C-kit in mouse with blood-deficiency constipation
摘要
Abstract
Objective To study the effects of Angelica sinensis radix (Danggui) on gastrointestinal hormone and expresomatostatinion of tyrosine kinase receptor C-kit in mouse with blood-deficiency constipation (Xuexu Bianmi).Methods Kunming mouse were randomly divided into six groups,each group had 10 mouse:normal group,model group,control group,three dose of experimental groups.Except the normal group,the mouse in rest groups were orally administrated every day with diphenoxylate 25mg · kg-1 and subcutaneously injected at 1,4,7,10 d with acetylphenyhydrazine 60 mg · kg-1 and intraperitoneally injected at 10-14 d with cyclophosphamide 40 mg · kg-1 to copy Xuexu Bianmi model.At the 14 d,normal and model group were administered physiological saline,the control group were administered solution of Changtongshu Keli 5 mg · kg-1,three dose of experimental groups were administered with different doses of Danggui decoction (4.17,8.33,16.67 g · kg-1).The levels of motilin(MLT),substance P(SP),vasoactive intestinal peptide(VIP),cholecystokinin-8 (CCK-8) and somatostatin in blood were measured by enzyme-linked immunosorbent(ELISA).The expression of C-kit in colon were measured by immunohistochemical technique.Results MLT (pg · mL-1) in normal group,model group,control group,three dose of experimental groups were 1830 ± 246,1498 ± 198,1722 ± 237,1697 ± 245,1754 ± 199,1865 ± 259,respectively;the SP (ng · L-1) in the 6 groups were 266 ± 30,222 ± 34,259 ± 32,218 ± 28,252 ± 29,268 ± 30,respectively;the VIP (ng · L-1) in the 6 groups were 435 ± 109,579 ± 107,508 ± 105,417 ± 139,413 ± 126,445 ± 101,respectively;CCK-8 (pmol · L-1) in the 6 groups were 229 ±26,138 ± 12,180 ±22,164 ± 16,159 ±26,210 ±31,respectively;SS (n · L-1) in the 6 groups were 100 ±20,138 ±29,93 ± 16,104 ±23,101 ±22,99 ± 16,respectively.Comparison between model group and normal group,the di-fference had significantly (P ＜ 0.01,P ＜ 0.05).Comparison between control group on the CCK-8,SS and model group,the difference had significantly(all P ＜0.01).Comparison between experimental-L group on the VIP,CCK-8,SS and model group,the difference had significantly (all P ＜ 0.05)Comparison between experimental-M group on the MLT,VIP,SS and model group,the difference had significantly (all P ＜ 0.05).Comparison between experimental-H group on the MLT,SP,VIP,CCK-8,SS and model group,the difference had significantly(P ＜0.05,P ＜ 0.01).Comparison with the model group,the expression of C-kit in colon in three doses experimental groups were increased.Conclusion The mechanism of Danggui in treating mouse woth Xuexu Bianmi may be related to adjusted gastrointestinal hormone in serum and colon and increased the expression of C-kit in colon.关键词
当归/血虚便秘/胃肠激素/C-kit表达Key words
Angelica sinensis/blood-deficiency and constipation/gastrointestinal hormone/C-kit expression分类
医药卫生引用本文复制引用
杜丽东,牛亭惠,吴国泰,刘五州,邵晶,任远..当归对血虚便秘模型小鼠胃肠激素与酪氨酸激酶受体C-kit表达的影响[J].中国临床药理学杂志,2018,34(10):1195-1198,4.基金项目
甘肃省中药药理与毒理学重点实验室开放基金资助项目(ZDSYS-KJ-2015-009,ZDSYS-ZZ-2015-002) （ZDSYS-KJ-2015-009,ZDSYS-ZZ-2015-002）
甘肃省中药现代制药工程研究院基金资助项目(YWW-2015050) （YWW-2015050）
陇原青年创新人才扶持计划基金资助项目(甘组电明字[2015]34号) （甘组电明字[2015]34号）
