中国临床药理学杂志2018，Vol.34Issue(10)：1226-1229,4.DOI:10.13699/j.cnki.1001-6821.2018.10.025
高效液相色谱-质谱联用法测定人血浆中依普利酮浓度
Determination of eplerenone in human plasma by HPLC-MS/MS
刘洪川 1李鹏飞 1杜萍 1赵瑞 1于伟越 1刘丽宏1
作者信息
- 1. 首都医科大学附属北京朝阳医院药事都,北京100020
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chromatography-tandem mass spectrometry method (HPLC-MS/MS) for the quantification and pharmacokinetic of eplerenone in human plasma.Methods The plasma was treated with protein precipitation,chromatographic column:Waters Symmetry C18 column (4.6 mm × 50 mm,5 μm),mobile phase:methanol-acetonitrile-1 mmol · L-1 ammonium acetate,gradient elution,column temperature:40 ℃,electrospray ionization (ESI) source was applied and operated in multiple reaction monitoring (MRM) mode via positive ionization.The specificity,lower limit of quantitation and standard curve,precision and recovery rate,matrix effect as well as the stability were investigated.Results A good linearity of eplerenone was obtained in the concentration range of 5.00-2000.00 ng · mL-1,with the lower limit of quantitation of 5.00 ng · mL-1.The method validation met the criteria for bio-samples determination.The standard curve of bicalutamide was y =2.95 × 10-2x + 1.46 × 10-2 (r =0.997 2).Intra-day and inter-day RSD of low,medium and high quality control samples were all less than 10％,the average recoveries of them were between 99.76％-100.87％,and stability was good.Conclusion The method was simple,rapid,sensitive and accurate,specific,suitable for the determination of eplerenone in human plasma.关键词
依普利酮/高效液相色谱-质谱联用法/药代动力学/血药浓度Key words
eplerenone/HPLC-MS/MS/pharmacokinetics/plasma concentration分类
医药卫生引用本文复制引用
刘洪川,李鹏飞,杜萍,赵瑞,于伟越,刘丽宏..高效液相色谱-质谱联用法测定人血浆中依普利酮浓度[J].中国临床药理学杂志,2018,34(10):1226-1229,4.
