中国免疫学杂志2018，Vol.34Issue(6)：810-814,5.DOI:10.3969/j.issn.1000-484X.2018.06.002
氯喹对正常胃上皮细胞GES-1和胃癌细胞HGC-27凋亡的不同影响
Inconsistent effect of chloroquine on apoptosis of normal gastric epithelial cells GES-1 and gastric cancer cell line HGC-27
摘要
Abstract
Objective:To compare the effect of chloroquine on apoptosis of normal gastric epithelial cells and gastric cancer cell line HGC-27. Methods:Change of these two kinds of cells were observed by inverted microscope after treating with CQ. HGC-27 cells were detected on the effect of apoptosis by DAPI nuclear staining after treating with CQ. The proliferation of cells were measured by CCK-8. Changes of mitochondrial membrane potential were investigated by JC-1 after treating with CQ. The expression of apoptosis protein effector enzyme Caspase-3 and substrate PARP in these two kinds of cells were tested by Western blot after using chloroquine (CQ) and rapamycin ( rapamycin, RAP ) to treat cells 72 h. Results: After treated with 10 μmol/L CQ 72 h, morphological characteristics of GES-1 cells and HGC-27 cells could be visible under the microscope,CQ induced apoptosis of GES-1 cells,on the contrary,it could make the HGC-27 cell get widened,the number of apoptotic cells gradually increased,the cell density decreased,cell atrophy and gradually turned round,cytoplasm reduced,at last,lose normal cell morphology. After two kinds of cells treated with CQ 72 h,as for GES-1 cell nuclei stained light,nuclear size and shape were not changed,however,HGC-27 nuclei showed pyknosis or granular fluorescence dense concentrated form. CCK-8 results showed that comparing with normal gastric epithelial cells GES-1,the pro-liferation of gastric cancer HGC-27 cells activity could be inhibited by CQ. JC-1 results showed that the change of the red fluorescence to green fluorescence in HGC-27 cells treated by CQ. Western blot showed that after being treated with CQ and RAP in normal gastric epithelial cells and HGC-27 cell line 72 h,the expression of apoptosis protein Caspase-3 and PARP in gastric cancer cell HGC-27 decreased significantly,comparing to that in GES-1 cells. Conclusion:Compared to normal gastric epithelial cells,CQ can inhibit human gastric cancer HGC-27 cell viability and induce apoptosis.关键词
氯喹/凋亡/GES-1细胞/HGC-27细胞Key words
Chloroquine/Apoptosis/GES-1 cells/HGC-27 cells分类
医药卫生引用本文复制引用
曹丽,苏强,张亚萍,张鑫艳,程凯,张晓延..氯喹对正常胃上皮细胞GES-1和胃癌细胞HGC-27凋亡的不同影响[J].中国免疫学杂志,2018,34(6):810-814,5.基金项目
本文受国家自然科学基金(No. 81301426)、山西医科大学汾阳学院校内科研项目(No. 2018C01)和山西省高等学校大学生创新创业训练项目(No. 2014499)资助. （No. 81301426）
