中国免疫学杂志2018，Vol.34Issue(6)：850-853,4.DOI:10.3969/j.issn.1000-484X.2018.06.010
姜黄素抑制CD44+结肠癌细胞增殖的体外实验研究
Proliferation inhibition effects of curcumin against CD44+colon cancer cells in vitro
摘要
Abstract
Objective:To study confirm whether curcumin could inhibit the proliferation of CD44+colon cancer cells. In this study. Methods: MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells. And Real-time PCR and Fluorescence-activated cell sorting (FACS) were used to determine CD44 expression level in colon cancer cells after treated with curcumin. Then we used Magnetic-activated cell sorting ( MACS) method to separate CD44 positive and negative cells. And we compared the proliferation inhibition effects of curcumin against these two kinds of cells. Results: Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines. The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin. Conclusion: Curcumin could inhibit the proliferation of CD44+colon cancer cells. Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.关键词
姜黄素/结肠癌/肿瘤干细胞/CD44+Key words
Curcumin/Colon cancer/Cancer stem cells/CD44+分类
医药卫生引用本文复制引用
郗雪艳,付飞,樊旭,杜伯雨..姜黄素抑制CD44+结肠癌细胞增殖的体外实验研究[J].中国免疫学杂志,2018,34(6):850-853,4.基金项目
本文为国家自然科学基金应急管理项目(81650019)和湖北省科技厅面上项目(2017CFB551,2017CFB462). （81650019）
