中国实验动物学报2018，Vol.26Issue(3)：265-271,7.DOI:10.3969/j.issn.1005-4847.2018.03.001
戊己丸对炎症后肠易激综合征大鼠脑肠肽的调控作用
Regulatory effect of a Chinese medicine Wuji Pill ( WJW) on brain-gut peptides in the rats of post-inflammatory irritable bowel syndrome
摘要
Abstract
Objective To explore the biological indicators of diagnosis and treatment of irritable bowel syndrome (IBS), and to explore the mechanism of action of a Chinese medicine Wuji Pill (WJW) on irritable bowel syndrome (IBS). Methods (1) Postinflammatory irritable bowel syndrome (PI-IBS) rat model was established by acetic acid plus restraint stress method . (2) The colonic motor ability of rats was evaluated by colon motility index (MI), the number of fecal particles discharged within 2 h, and the time of glass pellet discharge. (3) The formation of PI-IBS model rats and the therapeutic effect of WJW were observed. (4) The levels of calcitonin gene-related peptide (CGRP), motilin (MTL), neuropeptide Y (NPY), substance P (SP), somatostatin (SS), vasoactive intestinal peptide (VIP), and cholecystokinin (CCK) in the brain and colon tissues of PI-IBS rats were measured by ELISA. Results (1) The rat PI-IBS model was successfully established. Compared with the normal group, the body weight of the model rats was decreased, the food intake decreased, the amount of feces increased, loose stools and amorphous soft stools appeared, voluntary movements decreased, colon motility index ( MI) significantly increased ( P ＜ 0. 05 ), the number of fecal particles discharged significantly increased ( P＜ 0. 05), and the glass pellet discharge time was significantly shortened ( P ＜ 0. 05). (2) WJW treatment for 7 days significantly improved a variety of symptoms. Compared with the normal control, the levels of CGRP, SS and VIP in the brain tissue of PI-IBS rats were significantly increased (P＜ 0. 05), and the NPY concentration was significantly decreased ( P ＜ 0. 05). However, the treatment with WJW significantly reduced CGRP, SS and VIP levels (P＜ 0. 05), and significantly increased the NPY concentration level (P ＜ 0. 05). (3) Compared with the normal control group, the levels of CCK, NPY, MTL, SS and VIP in colonic tissues of PI-IBS rats were significantly decreased (P＜ 0. 05), while WJW significantly increased the CCK and VIP levels. Conclusions WJW can be used to treat IBS by regulating the levels of various brain-gut peptides in the brain and colon tissues of IBS rats. These anomalous and adjustable brain-gut peptides may become a potential biomarker for the diagnosis and treatment of IBS.关键词
肠易激综合征/脑肠肽/戊己丸/大鼠Key words
irritable bowel syndrome/brain-gut peptides/Wuji pill(a Chinese medicine)/rats分类
生物科学引用本文复制引用
黄鹤飞,朱晓新,陈颖,强伟杰,杨庆,李玉洁,翁小刚,王娅杰,李琦,蔡维艳,陈涛..戊己丸对炎症后肠易激综合征大鼠脑肠肽的调控作用[J].中国实验动物学报,2018,26(3):265-271,7.基金项目
国家自然科学基金项目(No. 81403171) （No. 81403171）
"重大新药创制"科技重大专项(No. 2017ZX09101002-002-008) （No. 2017ZX09101002-002-008）
中国中医科学院自选课题(No. QZPT001).Funded by National Natural Science Foundation of China(No. 81403171), Major New Drug Creation Foundation of China(No. 2017ZX09101002-002-008)and Autonomous Program of China Academy of Chinese Medical Sciences (No. QZPT001). （No. QZPT001）
