中国实验动物学报2018，Vol.26Issue(3)：272-279,8.DOI:10.3969/j.issn.1005-4847.2018.03.002
利用功能基因PCR芯片探讨过表达Slit2基因与老年小鼠淀粉样蛋白产生沉积的关系
The relationship between overexpression of Slit2 and accumulation of amyloid-β in aging mice studied by PCR array
摘要
Abstract
Objective Previous studies suggested that overerpression of Slit2 results in abnormal Alzheimer's disease-like behavior and cognition impairment in mice. The aim of this study is to investigate the relationship between overerpression of Slit2 and accumulation and clearance of amyloid-β in aging mice by comparing the differential expression of genes for accumulation and clearance of amyloid-β in aging Tg-Slit2 and Tg-2576 mice. Methods 14-month old male C57BL/6, Tg-Slit2 and Tg-2576 mice were used to detect the expression of Aβ1 - 40 and Aβ1 - 42 in brain by immunohistochemistry. Further, the total RNA in the brain of these mice were extracted, identified and inversely transcripted to cDNA, then the cDNA was detected by PCR array. The expression of genes in the brain of Tg-Slit2 and Tg-2576 mice were analyzed. Results Comparing with the Tg-2576 mice in the same age, accumulation of Aβ was not found in the brain of Tg-Slit2 and C57BL/6 mice. The result from PCR array analysis showed that comparing with the same aged C57BL/6 mice, there were 16 up-regulated genes and 8 down-regulated genes in the brain of Tg-Slit2 mice and 14 up-regulated genes and 17 down-regulated genes in the brain of Tg-Slit2 mice. The expression of amyloid beta precursor protein (APP) in the brain of the three group mice was not changed. The expression of presenilin 2 ( Psen2) related with Aβ production was significantly up-regulated in the Tg-2576 mice. In addition, the expression of low density lipoprotein receptor-related protein ( LRP) 6 and 9 were markedly decreased in the Tg-2576 mice. Notably, these genes were not changed in the brain of the aging Tg-Slit2 mice. Conclusions The accumulation of Aβ in the brain are not found in 14-month Tg-Slit2 mice, In addition, different from Tg-2576 mice, the significant changes of expression of Aβ-related genes is not found in the brain of Tg-Slit2 mice.关键词
Tg-Slit2小鼠/Tg-2576小鼠/功能基因PCR芯片/β淀粉样蛋白Key words
Tg-Slit2 mice/Tg-2576 mice/PCR array for functional genes/amyloid-β分类
生物科学引用本文复制引用
李舸,李航,刘书华,李韵峰,关雅伦,李雪娇,黄韧,张钰..利用功能基因PCR芯片探讨过表达Slit2基因与老年小鼠淀粉样蛋白产生沉积的关系[J].中国实验动物学报,2018,26(3):272-279,8.基金项目
国家自然科学基金项目(No. 31472061,No. 31702074) （No. 31472061,No. 31702074）
广东省科技计划项目(No. 015A020212027,No. 2016A030313790,No. 2017A040406007,No. 2017B030314171,No. 2017A070702001).Funded by National Natural Science Foundation of China (No. 31472061, No. 31702074) and Guangdong Provincial Science&Technology Project (No. 2015A020212027,No. 2016A030313790,No. 2017A040406007,No. 2017B030314171, No. 2017A070702001). （No. 015A020212027,No. 2016A030313790,No. 2017A040406007,No. 2017B030314171,No. 2017A070702001）
