中国实验动物学报2018，Vol.26Issue(3)：287-295,9.DOI:10.3969/j.issn.1005-4847.2018.03.004
1α,25(OH)2D3对大鼠关节软骨细胞蛋白聚糖和蛋白聚糖酶代谢的调节作用
Role of 1α,25(OH)2D3in the metabolism of aggrecan and aggrecanase in chondrocytes of rat articular cartilage
摘要
Abstract
Objective To investigate the role of vitamin D in the synthesis and degradation of aggrecan in rat articular chondrocytes at cellular level. Methods Rat articular chondrocytes were stimulated by IL-1α, IL-1β and TNF-α, respectively. Normal and inflammatory chondrocytes were treated with different doses of vitamin D, respectively. CCK8, Flow cytometry, real time-PCR and western blot analysis were used to examine the proliferation activity and apoptosis level of chondrocytes, and the expression of aggrecan, ADAMTS-4 and ADAMTS-5 at both mRNA and protein levels. Results IL-1α,IL-1β and TNF-α significantly decreased the proliferation activity and increased the apoptosis level of the chondrocytes. Furthermore, IL-1α, IL-1β and TNF-α significantly decreased the expression of aggrecan, and increased the expressions of ADAMTS-4 and ADAMTS-5 at both mRNA and protein levels in the chondrocytes. 1α,25 (OH)2D3supplementation significantly increased the proliferation activity and decreased the apoptosis level of chondrocytes stimulated by IL-1α, IL-1β and TNF-α in a dose-dependent manner, but not affected the normal chondrocytes. Meanwhile, 1α,25(OH)2D3also significantly increased the expression of aggrecan, and decreased the expressions of ADAMTS-4 and ADAMTS-5 at both mRNA and protein levels in the chondrocytes under inflammatory conditions. Conclusions Vitamin D may promote the anabolism of aggrecan and inhibit aggrecanase activity in chondrocytes under inflammatory conditions, which may impact overall protection for articular cartilage.关键词
维生素D/蛋白聚糖/蛋白聚糖酶/软骨细胞/骨关节炎Key words
1α/25(OH)2D3/aggrecan/aggrecanase/chondrocytes/osteoarthritis分类
生物科学引用本文复制引用
官剑,谭启钊,赵振达,刘忠军,宋纯理,冷慧杰..1α,25(OH)2D3对大鼠关节软骨细胞蛋白聚糖和蛋白聚糖酶代谢的调节作用[J].中国实验动物学报,2018,26(3):287-295,9.基金项目
国家自然科学基金(No. 11472017,No. 11002004).Funded by National Natural Science Foundation of China (No. 11472017,No. 11002004). （No. 11472017,No. 11002004）
