中国实验动物学报2018，Vol.26Issue(3)：302-310,9.DOI:10.3969/j.issn.1005-4847.2018.03.006
Slit2基因过表达小鼠的生物学参数及心脏生理功能分析
Biological parameters and cardiac physiological function of the mice overexpressing human Slit2 gene
摘要
Abstract
Objective The basic biological, echocardiography and gene sequencing parameters of mice overexpressing Slit2 gene (Slit2-Tg mice) were collected and evaluated, and to provide a reference for the application of Slit2-Tg mice in biomedical research. Methods Slit2-Tg and C57BL/6 J mice were inbred. The genotypes of the mice were determined by a PCR assay. The blood samples were collected for blood routine and biochemical tests. The tissues of main organs were collected for protein expression and pathological analysis. Echocardiography and transcriptome sequencing was carried out for analyzing the heart function and gene expression, respectively. Results The litter size was significantly higher in the Slit2-Tg mice than in C57BL/6 J mice. Human Slit2 gene and protein expressions were detected in the main organs of Slit2-Tg mice. Organ coefficient of spleen was significantly increased in Slit2-Tg mice, but the tissue structure appeared normal. There were significant changes in the counts of erythrocytes, platelets, eosinophils, and biochemistry of glucose, globulin, urea nitrogen, triglycerides, HDL, and atherosclerosis index. Echocardiography showed no significant differences in the morphology and function of the Slit2-Tg hearts except in the left ventricular anterior wall thickness at the end-diastolic state. Compared with the C57BL/6 J mice, 535 genes out of 17513 genes in the Slit2-Tg hearts were increased or decreased, mainly involving 15 biological process or signal transduction pathways. Conclusions This study has collected the biological parameters of Slit2-Tg mice and suggests that this model animal is suitable for the studies of cardiovascular diseases.关键词
Slit2蛋白/模式动物/生物学参数/转录组测序分析/心血管疾病Key words
Slit2 protein/model animal/biological parameters/transcriptome sequencing/cardiovascular disease分类
生物科学引用本文复制引用
李想,杨丰华,黄韧,郑凌云,郑双,谭伟江,王静,黎冰林,罗挺,李舸,王丽京..Slit2基因过表达小鼠的生物学参数及心脏生理功能分析[J].中国实验动物学报,2018,26(3):302-310,9.基金项目
广东省科技专项(No. 2017B020231001,No. 2017A070702001,No. 2016A020216019) （No. 2017B020231001,No. 2017A070702001,No. 2016A020216019）
国家自然科学基金-面上项目(No. 31672376,No. 31571178) （No. 31672376,No. 31571178）
广东省实验动物重点实验室(No. 2017B030314171).Funded by National Natural Science Foundation of China (No. 2017B020231001, No. 2017A070702001, No. 2016A020216019), National Natural Science Foundation of China-Surface Projects (No. 31672376, No. 31571178), Guangdong Key Laboratory for Laboratory Animals (No. 2017B030314171). （No. 2017B030314171）
