中国实验动物学报2018，Vol.26Issue(3)：335-342,8.DOI:10.3969/j.issn.1005-4847.2018.03.011
EV71不同临床株的新生小鼠毒力研究
Virulence of enterovirus 71 in infected neonatal mouse models
摘要
Abstract
Objective To explore the virulence of enterovirus 71 from infected children in neonatal mice. Methods Three strains of EV71 were isolated from the mild, severe and dead patients. Symptoms, weight and death of mice were recorded throughout 14 days. The mice were sacrificed on the first, third, fifth, seventh and ninth days post infection to gain the tissue virus load including the liver, spleen, lung, intestine, brain and muscle tissue which were used to detect the virus tilter by real-time RT-QPCR, and pathological lesions using HE staining. Results As to the severity of symptoms, no significant difference was found between the severe and mild groups (P＝0. 693), which were more serious than that of the fatal group. (P＝0. 000 ＜ 0. 05/6, P＝0. 000 ＜ 0. 05/6). The survival rate of the mice with mild, severe and fatal virus infection was 77. 2%, 81. 7% and 97. 8%, respectively, and there was a significant difference among the three groups (P＝0. 0010 ＜ 0. 05, P＝0. 001 ＜ 0. 05, P＝0. 0004 ＜ 0. 05). Lung hemorrhage of the mild group was the most serious, and there were no significant differences in pathological lesions of the brain, muscle, spleen and intestine. Virus titer in the liver and muscle was higher than the other tissues and that in mild group of different tissues tended to be higher than the other two groups. Conclusions Neonatal mice infected with the mild strain of enterovirus 71 presents heaviest symptoms, which are not consistent with the outcomes of humans. It is considered to be related to the virus gene, host and other factors.关键词
EV71/感染/毒力/小鼠Key words
enterovirus 71/infection/virulence/mouse分类
生物科学引用本文复制引用
李怡沅,陈恒,苗瑞雪,李伟然,程悦,万朝敏,朱渝..EV71不同临床株的新生小鼠毒力研究[J].中国实验动物学报,2018,26(3):335-342,8.基金项目
四川省科技厅项目(No. 2014JY0003,No. 2017JY0089) （No. 2014JY0003,No. 2017JY0089）
市科技局项(No. 2015-HM01-00015-SF,No. 20160280,No. 2015041).Funded by Science and Technology Department of Sichuan Province(No. 2014JY0003, No. 2017JY0089), Science and Technology Bureau of Chengdu(No. 2015-HM01-00015-SF, No. 20160280, NO. 2015041). （No. 2015-HM01-00015-SF,No. 20160280,No. 2015041）
