中国实验动物学报2018，Vol.26Issue(3)：365-371,7.DOI:10.3969/j.issn.1005-4847.2018.03.015
一种体外皮肤腐蚀性试验替代方法的建立
Verification and application of the rat skin transcutaneous electrical resistance test ( TER) as an alternative method to replace the animal skin corrosion test
罗飞亚 1张露勇 1邢书霞 1王钢力1
作者信息
- 1. 中国食品药品检定研究院,北京 100050
- 折叠
摘要
Abstract
Objective In order to verify an alternative method for the skin corrosion test by using transcutaneous electrical resistance ( TER) test, and to optimize the implementation criteria in OECD TG 430 procedure. Methods According to the OECD TG 430 procedure, Wistar rat skin was used to test the TER values of 16 reference chemicals, and selected the most optimal standard via different implementation criteria. The program B was chosen to make inter-laboratory comparison between 5 laboratories by testing 11 chemicals, which were identified as the optimal standard. Results After the TER test, the result of corrosion test of 16 chemicals were accordant with the reference data ( Kappa value＝0. 64). The program B was the most optimal implementation criteria, and the specificity was 66. 7% and sensitivity was 100%. There were no significant differences between the corrosion estimations of 5 laboratories, and the concordance rate of the 5 laboratories was 72. 7%. Conclusions Transcutaneous electrical resistance (TER) test is an feasible and efficient tool for skin corrosion testing, and may become a good interim test to replace the in vivo test with this ex vivo test in cosmetics chemical safety assessment, thus, to reduce the animal usage in our country.关键词
大鼠经皮电阻试验/皮肤腐蚀性/替代方法/安全性评价/离体皮肤Key words
transcutaneous electrical resistance/TER/skin corrosion/animal alternative methods/safety assessment/ex vivo skin分类
生物科学引用本文复制引用
罗飞亚,张露勇,邢书霞,王钢力..一种体外皮肤腐蚀性试验替代方法的建立[J].中国实验动物学报,2018,26(3):365-371,7.
