中国比较医学杂志2018，Vol.28Issue(1)：33-37,5.DOI:10.3969/j.issn.1671-7856.2018.01.006
不同性别氟中毒大鼠模型的比较研究
Comparison of gender differences in the rat model of fluorosis
摘要
Abstract
Objective To construct the male and female rat model of fluorosis and to explore the gender difference in fluorosis. Methods A total of 48 clean-grade SD rats with the body weight of about 150 g (male∶female=1∶1) were equally divided into the control group and fluorosis model group. Rats in the fluorosis model group were fed with fluorine-containing (100 mg/kg) feedstuff. The general condition, changes in body weight and dental fluorosis of the rats were observed every three days. A batch of female and male rats were sacrificed by femoral artery bleeding on the 70th and 110th day of experiment, respectively, when all rats showed dental fluorosis. The levels of urinary fluoride and bone fluoride of the rats were measured by a fluoride ion-selective electrode. Results All the male and female rats in the control group did not show dental fluorosis during the entire experiment. The female and male rats in the fluorosis group showed dental fluorosis visible to naked eye began approximately on the 60th and 80th day, respectively, indicating that the appearance of dental fluorosis of the male rats was about 20 days later than the female rats. After 30 days or so, the male and female rats went into a rapid growth period, and there were significant differences between the body weights of the male and female rats (P＜ 0. 01). The body weight of the male and female rats in the fluorosis group was higher than that of the control group on the 110th day. With the progression of fluorosis, the levels of urinary fluoride and bone fluoride of the rats were gradually increased, and finally significantly higher than those of the control group (P＜ 0. 01). There was a positive correlation between the levels of urinary and bone fluoride and the time of fluorosis. The levels of urinary and bone fluoride of the male rats were significantly higher than those of the female rats (P＜ 0. 01), indicating that they are affected by gender. Conclusions The body weight of the rat model of fluorosis, the starting time when dental fluorosis occurs and the levels of urinary and bone fluorine are all different between male and female rats.关键词
氟中毒/氟斑牙/体重/动物模型/大鼠Key words
fluorosis/dental fluorosis/body weight/animal model/rats分类
医药卫生引用本文复制引用
刘洋,韩伦英,何川,彭凤涛,李桂筑,王飞清..不同性别氟中毒大鼠模型的比较研究[J].中国比较医学杂志,2018,28(1):33-37,5.基金项目
贵州省科技厅项目(黔科合LH字[2016]7511号) （黔科合LH字[2016]7511号）
贵州省教育厅青年科技人才成长项目(黔教合KY [2016]190). （黔教合KY [2016]190）
