中国比较医学杂志2018，Vol.28Issue(1)：64-69,6.DOI:10.3969/j.issn.1671-7856.2018.01.011
Tempol对心肌肥厚大鼠NF-κB信号通路的影响
Effect of tempol on NF-κB signaling pathway in the rat model of myocardial hypertrophy
摘要
Abstract
Objective To investigate the effects of 2, 2, 6, 6-tetramethyl-4-piperidinol (tempol) on NF-κB signaling pathway of myocardial hypertrophy in rats. Methods The rat model of myocardial hypertrophy was established by intraperitoneal injection of isoprenaline (ISO) (5 mg/kg, twice per day, 2 weeks). A total of 42 male SD rats were randomly divided into 3 groups, including the control group, myocardial hypertrophy model group (ISO + sterile saline) and tempol treatment group (ISO + tempol) [tempol 100 mg/ (kg·d), 8 weeks]. Eight weeks after the corresponding drug intervention, the heart weight index (HWI) and left ventricular weight index (LVWI) were determined. Morphology and fibrosis of the myocardium were observed by HE staining, and the myocardial fibrosis of the rats was observed by Masson staining. The mRNA levels of TNF-α and IL-6 in the rat myocardial tissues were detected by qRT-PCR, and the expression levels of IκBα, p-p65 and p65 were detected by Western blot. Results Compared with the control group, the HWI and LVMI, mRNA levels of TNF-α and IL-6, and expression of p-p65/p65 in the model group were significantly increased (P＜ 0. 05), while the expression level of IκBα, an NF-κB inhibitory protein was significantly decreased (P＜0. 05). The pathological examination of the myocardial tissues showed thickening and disordered arrangement of myocardial fibers, and increased cross-sectional area of the myocardial fibers. The pathology by Masson staining showed aggravated myocardial fibrosis and increased collagen fibers in the myocardial interstitium. Compared with the model group, the HWI and LVMI, the mRNA levels of TNF-α and IL-6, and the expression of p- p65/p65 of the tempol group were significantly decreased (P＜ 0. 05), while the expression level of IκBα was significantly increased (P＜ 0. 05). HE staining showed that the degrees of myocardial disarrangement and cardiomyocyte hypertrophy were decreased. Meanwhile, Masson staining showed that the extent of myocardial fibrosis was reduced, and the interstitial collagen fibers were decreased. Conclusions Tempol can improve the isoprenaline-induced myocardial hypertrophy, which may be closely related with the inhibition of the activity of NF-κB signaling pathway.关键词
NF-κB信号通路/2,2,6,6-四甲基-4-哌啶醇/心肌肥厚/异丙肾上腺素Key words
NF-κB signaling pathway/2/2/6/6-tetramethyl-4-piperidinol/tempol/myocardial hypertrophy/isoprenaline分类
医药卫生引用本文复制引用
刘捡娣,谢龙,肖坤,朱金海,谢东明..Tempol对心肌肥厚大鼠NF-κB信号通路的影响[J].中国比较医学杂志,2018,28(1):64-69,6.基金项目
赣南医学院研究生创新专项资金项目(编号: YC2016-X014). （编号: YC2016-X014）
