中国比较医学杂志2018，Vol.28Issue(1)：108-112,5.DOI:10.3969/j.issn.1671-7856.2018.01.019
针刺治疗慢性心肌缺血小型猪模型系列方法的可行性探讨
Feasibility study of acupuncture treatment on the miniature pig model of chronic myocardial ischemia
摘要
Abstract
Objective To explore the feasibility of needle-embedding therapy in the treatment of chronic myocardial ischemia using a miniature pig model established by placement of an Ameroid constrictor at the left anterior descending branch (LAD) of coronary artery in Bama miniature pigs during surgery. Methods The miniature pig model of chronic myocardial ischemia was established by placement of an Ameroid constrictor at the left anterior descending branch of the left coronary artery in Bama miniature pigs. The pig models were randomly divided into the treatment group (the"Neiguan " group) and the control group (the "Zusanli " group), and were treated with needle- embedding electroacupuncture at the"Neiguan" (PC6) and "Zusanli" (ST36) acupoints, respectively. Myocardial samples were taken at 6 weeks after surgery for light and electron microscopic examinations. Results Gross pathology showed that ischemic area in the myocardium appeared in both experimental groups. The ischemic area in the "Zusanli "group was larger than that of the"Neiguan"group. Histopathology showed that the acupuncture treatment at the"Neiguan"acupoint reduced the ischemic injury in the pig myocardial tissues. Ultrastructural observation of the myocardium showed mitochondrial vacuolization in cardiomyocytes and myocardial fibrosis in both groups. Conclusions Acupuncture therapy at the"Neiguan"acupoint of pericardial channel may exert protective effect on the myocardial ischemia by reducing the ischemia-injury of cardiomyocytes, but can not inhibit the already existed ischemia-induced cardiomyocytic injuries. Our findings suggest that the establishment of miniature pig model of chronic myocardial ischemia by surgically placing an Ameroid constrictor on the left anterior descending branch of left coronary artery and the needle-embedding in acupoints is feasible for the treatment of chronic myocardial ischemia in this pig model.关键词
大型实验动物/巴马小型猪/慢性心肌缺血/埋针并电针/大体病理/电镜超微结构Key words
large laboratory animals/Bama miniature pigs/chronic myocardial ischemia/needle-embedding electroacupuncture/gross pathology/electron microscopic ultrastructure分类
医药卫生引用本文复制引用
张金铃,孟宏,田毅,荣培晶,方继良,王瑜,石力,贾术永,何勋,雷洪涛..针刺治疗慢性心肌缺血小型猪模型系列方法的可行性探讨[J].中国比较医学杂志,2018,28(1):108-112,5.基金项目
国家重点基础研究发展计划 (973计划)项目(编号: 2012CB518503) （973计划）
国自然青年基金(编号: 81102650) （编号: 81102650）
北京工商大学北京市植物资源研究开发重点实验室开放课题基金资助课 题. （）
