中国比较医学杂志2018，Vol.28Issue(6)：4-9,6.DOI:10.3969/j.issn.1671-7856.2018.06.002
A型肉毒素重链干预神经细胞组蛋白3乙酰化水平的初步研究
Botulinum neurotoxin serotype A heavy chain intervenes in the H3 acetylation: A preliminary study
摘要
Abstract
Objective To investigate the effect and molecular mechanism of botulinum neurotoxin serotype A (BoNT/A) heavy chain on neuron regeneration. Methods Cell culture, rats, immunofluorescence, SDS-PAGE and western blot, etc. were adopted in this study to explore the alterations of histone-3 acetylation (acetyl-H3 ) by local treatment of BoNT/A heavy chain to spinal cord injury (SCI) in rats (in vivo) or by adding it into cell culture (in vitro). Meanwhile, the relevance of acetyl-H3 to neurite out-growth based on SCI and cell culture with BoNT/A heavy chain application was approached as well. Results The application of BoNT/A heavy chain to cultured Neuro-2a cells increased the level of H3 acetylation. The increase of H3 acetylation was paralleled with the growth of neuritogenesis. Also, the neuronal treatment of BoNT/A heavy chain to SCI promoted the re-growth of neuronal processes surrounding the lesions. The growth of neuronal processes was positively correlated to the level of H3 acetylation. During the periods of BoNT/A heavy chain treatment in vivo or in vitro, the increase of H3 acetylation showed two peaks. Conclusions BoNT/A heavy chain increased the H3 acetylation, which might be one of its neuritogenic mechanisms.关键词
A型肉毒素/A型肉毒素重链/组蛋白乙酰化/乙酰化/去乙酰化/神经突起再生/脊髓损伤Key words
botulinum neurotoxin serotype A, BoNT/A/BoNT/A heavy chain/histone acetylation/acetylation/deacetylation/neuritogenesis/spinal cord injury, SCI分类
医药卫生引用本文复制引用
兰婧,刘福,高扬,李志强,刘雅,赵明伟,袁海霞,李夏青..A型肉毒素重链干预神经细胞组蛋白3乙酰化水平的初步研究[J].中国比较医学杂志,2018,28(6):4-9,6.基金项目
国家自然科学基金(81171178) （81171178）
山西医科大学大学生创新创业基金(20170105). （20170105）
