中国比较医学杂志2018，Vol.28Issue(6)：15-20,28,7.DOI:10.3969/j.issn.1671-7856.2018.06.004
实验用狨猴微卫星引物筛选及遗传多样性分析
Microsatellite marker design and genetic analysis for the common marmoset
摘要
Abstract
Objective To screen and optimize the microsatellite DNA primers of the laboratory common marmoset, analyze and evaluate the population genetic quality for the marmosets (Callithrix jacchus) introduced into the Institute of Medical Laboratory Animal science, Chinese Academy of Medical Sciences. Methods A total of 30 marmosets were randomly chosen, and their genome DNA from blood was extracted using phenol/chloroform method. The microsatellite DNA was amplified using standard polymerase chain reaction (PCR). The amplification products were tested by STR scanning after 2% agrose gel and 8% PAGE electrophoresis. The data processing and genetic analysis were completed using the Popgene1. 32 software. Results A total of 20 pairs of microsatellite loci showed genetic polymorphism, and 147 alleles were detected. The number of allele was 5 to 10, average 7. 35. The effective allele was 2. 2500 to 6. 3830, average 4. 0402. The observed heterozygosity was 0. 000 to 0. 4667, average 0. 1533. The expected heterozygosity was 0. 1424 to 0. 4350, average 0. 2506. The Shannon diversity index was 1. 2242 to 2. 0324, average 1. 5949. The polymorphic information content was 0. 5366 to 0. 8254, average 0. 7053. Conclusions The 20 pairs of marmoset microsatellite primers are genetically highly diverse and are in a Hardy-Weinberg equilibrium.关键词
实验用狨猴/微卫星DNA/遗传多样性Key words
marmoset/microsatellite DNA/genetic diversity分类
医药卫生引用本文复制引用
滕永康,刘先菊,张旭,向志光,阮研硕,肖冲,刘云波..实验用狨猴微卫星引物筛选及遗传多样性分析[J].中国比较医学杂志,2018,28(6):15-20,28,7.基金项目
国家重点研发计划(2017YFA0105201) （2017YFA0105201）
北京市科技计划(Z161100000216154). （Z161100000216154）
