中国比较医学杂志2018，Vol.28Issue(6)：21-28,8.DOI:10.3969/j.issn.1671-7856.2018.06.005
金线莲醇提物在斑马鱼中降血糖效果的探究
Exploration of the hypoglycemic effect of Anoectochilus roxburghii alcohol extracts on diabetic zebrafish models
摘要
Abstract
Objective The aim of this study is to explore the hypoglycemic effect of active components of Anoectochilus roxburghii on zebrafish models. Methods Anoectochilus roxburghii components were extracted and separated into three groups: the alcohol extraction group, macromolecular polysaccharide group (≥ 5 ×103) and small molecular polysaccharide group (＜5×103). Zebrafish embryos were exposed to 2% glucose solution (2% Glu) at 24 h to imitate acute hyperglycemia phenotype, and then treated with the three Anoectochilus roxburghii components. Based on this high-glucose model, the zebrafish embryos at 72 h were collected to detect the whole tissue glucose value. Furthermore, semi-quantitative PCR and whole mount in situ hybridization were performed to detect the expression of mRNA levels of glycometabolism-related genes. Results An acute diabetic zebrafish model was induced by high glucose stress. In this model, some key factors during glycometabolism such as insulin, pck-1 and pdx-1 were significantly affected, while the alcohol extracts of Anoectochilus roxburghii obviously reversed these abnormalities induced by high glucose stress, even to normal levels. Conclusions The alcohol extracts of Anoectochilus roxburghii has obvious hypoglycemic effect on diabetic zebrafish model. Our result suggest that Anoectochilus roxburghii has a potential application in the hypoglycemic drug screening.关键词
金线莲醇提物/斑马鱼/组织液葡萄糖/高糖模型/糖代谢基因Key words
Anoectochilus roxburghii alcohol extracts/zebrafish/tissue glucose/hyperglycemia model/glycometabolism-related genes分类
医药卫生引用本文复制引用
许璟瑾,张文娟,陈志永,李秀敏,潘裕添,王兵丽,欧一新,薛钰..金线莲醇提物在斑马鱼中降血糖效果的探究[J].中国比较医学杂志,2018,28(6):21-28,8.基金项目
福建省自然科学基金项目资助(2015J05070) （2015J05070）
福建省高校杰出青年科研人才培育计划项目 （）
福建省教育厅科技项目(JK2015028) （JK2015028）
福建省中青年教师教育科研项目(JA15318). （JA15318）
