中国比较医学杂志2018，Vol.28Issue(6)：101-105,5.DOI:10.3969/j.issn.1671-7856.2018.06.018
兔抗狨猴IgG-HRP标记抗体的制备及初步鉴定
Preparation and identification of rabbit anti-marmoset IgG-HRP conjugate antibody
摘要
Abstract
Objective To purify marmoset serum IgG, prepare and identify the antiserum and the rabbit anti-marmoset antibody IgG-HRP (horseradish peroxidase). Methods Using SDS-PAGE analysis to identify the serum IgG from HiTrapTM Protein G. The antiserum titer was determined by double immunodiffusion assay. The rabbit anti-marmoset IgG was labeled with HRP by improved sodium periodate method. ELISA and western blotting were used to evaluate the concentration and specificity of rabbit anti-marmoset IgG-HRP. Results The purity of purified marmoset serum IgG determined by SDS-PAGE was higher than 95% , and the anti-serum titer of the anti-marmoset IgG polyclonal antibody was 1∶64. The concentration of rabbit anti-marmoset IgG-HRP identified by direct ELISA was 1∶256 000, and that by western-blotting was 1∶15 000, with a strong specificity. Conclusions The IgG-HRP marker antibody is prepared and the specificity and concentration are identified by ELISA and western blotting. It reserves the resources for the detection system of marmoset pathogens and the molecular immunological testing system.关键词
狨猴/抗体制备/酶标抗体Key words
marmoset/preparation of antibodies/rabbit anti-marmoset IgG-HRP分类
医药卫生引用本文复制引用
丛日旭,刘先菊,滕永康,向志光,佟巍,张丽芳,阮研硕,刘云波..兔抗狨猴IgG-HRP标记抗体的制备及初步鉴定[J].中国比较医学杂志,2018,28(6):101-105,5.基金项目
北京市科技计划(Z161100000216154) （Z161100000216154）
国家重点研究计划(2017YFA0105201). （2017YFA0105201）
