中国比较医学杂志2018，Vol.28Issue(6)：106-110,5.DOI:10.3969/j.issn.1671-7856.2018.06.019
背入式腹膜后双肾蒂夹闭制作肾缺血再灌注损伤模型的技术方法
Establishment method of a rat model of renal ischemia-reperfusion injury by clamping the bilateral renal pedicles through backside and retroperitoneal incision
摘要
Abstract
Objective To explore a method to prepare a model of renal ischemia-reperfusion injury, with mild injury to animals, simple and easy to operate and stable effectiveness. Methods Healthy SD male rats were randomly divided into normal group (group C), sham-operated group (group S) and experimental group (IR group). Rats in the experimental group received a median incision in the middle of the back skin. Through the muscle fascia of each side on the back into the bilateral retroperitoneal space, the bilateral renal pedicles were separated, and closed for 50 min with a mini artery clamp without injury, and then the blood perfusion of the bilateral renal pedicles was resumed by releasing the mini artery clamp. Except that the rats of sham operation group were not blocked the renal pedicle, the rest of the operating steps were consistent with the experimental group. The normal group rats were not treated except for anesthesia. To observe the condition of the rats, serum creatinine (SCr), blood urea nitrogen (BUN) and pathologyical changes in the kidney tissues at 24 h after operating. Results Skin incision was 2. 24 ± 0. 27 cm in the group IR, the right incision of lumbar dorsal fascia and muscle was 1. 36 ± 0. 21 cm, and the left incision was 1. 36 ± 0. 24 cm. The operation time was 3. 30 ± 0. 37 min from incising skin to clamping the bilateral kidney pedicles. The successful rate of model preparation reached 95% in the IR group. Compared with the groups C and S, the levels of SCr and BUN were remarkably increased in the IR group (P ＜0. 01), and renal tubular necrosis scores were also significantly increased (P＜ 0. 05). Conclusions The rat model of renal ischemia-reperfusion injury is established by clamping bilateral renal pedicles via dorsal retroperitoneal incision. This surgical procedure is stable and simple, with a high success rate and small incision, less bleeding, and minimal animal injury.关键词
肾脏/动物模型/背部/腹膜后/缺血再灌注损伤Key words
kidneys/animal model/back/retroperitoneum/ischemia-reperfusion injury分类
医药卫生引用本文复制引用
谢敏慧,谷翠芝,李凤霞,李清初..背入式腹膜后双肾蒂夹闭制作肾缺血再灌注损伤模型的技术方法[J].中国比较医学杂志,2018,28(6):106-110,5.基金项目
广西高校科学技术研究项目(LX2014264) （LX2014264）
广西自然科学基金面上项目(2016JJA140396). （2016JJA140396）
