中国动物传染病学报2018，Vol.26Issue(3)：7-15,9.
蝙蝠流感病毒与H9N2亚型禽流感病毒M蛋白的免疫原性比较研究
COMPARATIVE STUDY ON IMMUNOGENICITY FOR M PROTEINS OF BAT INFLUENZA VIRUS AND H9N2 AVIAN INFLUENZA VIRUS
摘要
Abstract
The homology for M1 protein of the Bat influenza virus and other known Influenza A virus is about 78%, and the homology for M2 protein is much lower as 42%. To reveal the immunogenicity difference for M1 and M2 protein of the newly discovered Bat influenza virus, we constructed the prokaryotic and eukaryotic expression plasmids for the M1/M2 encoded proteins of the H17N10 Bat influenza virus (A/little yellow-shouldered bat/Guatemala/164/2009) and an H9N2 Avian influenza virus(A/chicken/Jiangsu/A2093/2011). Results showed that the M1 proteins of both viruses expressed by the prokaryotic plasmids were only able to react with the M1 polyclonal antibody, but not two monoclonal antibodies (5F2, 3G8), whereas the M1 proteins of both viruses expressed by the eukaryotic plasmids or the virus-infected cells only react with the 5F2 monoclonal antibody, not with the 3G8 and polyclonal antibodies. The data indicated the M1 proteins from both viruses displayed similar immunogenicities of tested antibodies. Interestingly, M2 polyclonal antibody could only react with the H9N2 M2 proteins expressed by eukaryotic plasmids or the virus-infected cells, not with Bat influenza virus. In contrast, the M2 monoclonal antibody(14C2) only reacted with the Bat influenza virus M2 expressed by the prokaryotic plasmids, not with the H9N2 M2 expressed by the prokaryotic plasmids. Our study demonstrated the different immunogenicities for M2 protein between Bat influenza and H9N2 Avian influenza viruses, and revealed that the M2 antibody could be used to distinguish these two viruses. Our study provided a basis for future research related to M proteins of both infuenza viruses.关键词
蝙蝠流感病毒/H9N2亚型禽流感病毒/M蛋白/免疫原性Key words
Bat influenza virus/H9N2 Avian influenza virus/M protein/immunogenicity分类
农业科技引用本文复制引用
黄敏,闫大为,周伟光,李泽君,杨健美,刘宸瑞,滕巧泱,刘芹防,李雪松,陈鸿军,车广胜,任超超,崔宏锐..蝙蝠流感病毒与H9N2亚型禽流感病毒M蛋白的免疫原性比较研究[J].中国动物传染病学报,2018,26(3):7-15,9.基金项目
国家自然科学基金项目(青年科学基金项目31700136) （青年科学基金项目31700136）
上海市兽医生物技术重点实验室开放基金项目(klab201706) （klab201706）
