中国动物传染病学报2018，Vol.26Issue(3)：16-22,7.
含有WSN病毒基因的重组H9N2亚型流感病毒的复制能力研究
REPLICATION OF REASSORTANT H9N2 INFLUENZA VIRUSES CONTAINING THE GENES OF WSN STRAIN
摘要
Abstract
Avian influenza virus poses a great threat to not only poultry, but also human beings. Several human pandemics resulted from the reassortant of avian and human influenza viruses. Four reassortant viruses, including r2093HANAPB2/WSN, r2093HANAPB1/WSN, r2093HANAPA/WSN and r2093HANANP/WSN, were rescued by reverse genetics, each of which contains the HA gene, NA gene, and one of polymerase genes or NP gene of A/Chicken/Shanghai/2093/2009, and other 5 genes of A/WSN/1933(H1N1). The replication capability of the reassortant viruses were tested on MDCK, DF1 and A549 cells in mice. The results showed that virus titers of all these reassortant viruses reached the peak on MDCK, DF1 and A549 cells infected with 0.001 multiplicity of infection (MOI) at 24 hours post infection, while r2093HANAPB1/WSN and r2093HANAPA/WSN replicated better than r2093HANAPB2/WSN and r2093HANANP/WSN in those cells. Accordingly, r2093HANAPB1/WSN and r2093HANAPA/WSN showed higher replication efficiency than r2093HANAPB2/WSN and r2093HANANP/WSN in nasal turbinates of mice inoculated intranasally, although the titers of those viruses did not show clear difference in the mouse lungs. It is suggested that the replication capabilities of the reassortants with different polymerase genes or NP gene are significantly different and the molecular mechanism causing the difference is worth to be explored further.关键词
禽流感病毒/H9N2亚型/病毒拯救/重组/复制Key words
Avian influenza virus/H9N2 subtype/virus rescue/reassortant/replication分类
农业科技引用本文复制引用
车广胜,李泽君,崔宏锐,屈梦锦,林伟山,滕巧泱,杨健美,刘芹防,陈鸿军,李雪松..含有WSN病毒基因的重组H9N2亚型流感病毒的复制能力研究[J].中国动物传染病学报,2018,26(3):16-22,7.基金项目
国家自然科学基金项目(31472206) （31472206）
