中国动物传染病学报2018，Vol.26Issue(3)：23-27,5.
欧洲类禽H1N1亚型猪流感病毒反向遗传操作平台的建立
ESTABLISHMENT OF REVERSE GENETICS SYSTEM OF EUROPEAN AVIAN-LIKE H1N1 SUBTYPE SWINE INFLUENZA VIRUS
摘要
Abstract
To rescue A/swine/TianJin/6/2013(H1N1) of Swine influenza virus, the eight gene segments of H1N1 were amplified by RT-PCR and cloned into the transcription/ expression vector of pBD. The eight recombinant plasmids were purified and cotransfect into 293T cells. The supernatant of 293T cells was collected after 54 hours and inoculated into MDCK cells. When cytopathic effects (CPE) appeared in MDCK cells, virus titer was determined at 1 : 32 in hemagglutination test. The full genome of the rescued virus had no nucleic acid change, compared with the wild-type virus through sequence analysis. There were no obvious differences in CPE on MDCK cells between the rescued virus and the wild type virus. The establishment of reverse genetic system of European avian-like H1N1 subtype Swine influenza virus provided the foundation for further studies on gene function and new influenza vaccine.关键词
猪流感病毒/病毒拯救/生长曲线/H1N1亚型Key words
Swine influenza virus/rescue virus/growth curve/H1N1 subtype分类
农业科技引用本文复制引用
汪琪,周艳君,李国新,郑浩,高飞,姜一峰,童光志,于海,杨海明,汪秀会,刘晓敏,王帅勇,姚云,朱世强,单同领,童武..欧洲类禽H1N1亚型猪流感病毒反向遗传操作平台的建立[J].中国动物传染病学报,2018,26(3):23-27,5.基金项目
上海市自然科学基金青年项目(16ZR1444000) （16ZR1444000）
中央级公益性科研院所基本科研业务费项目(2015JB07) （2015JB07）
中国农业科学院创新工程"猪病毒性繁殖障碍综合症团队"项目 （）
