中国动物传染病学报2018，Vol.26Issue(3)：34-39,6.
一株牛肠道病毒的分离与鉴定
ISOLATION AND IDENTIFICATION OF A BOVINE ENTEROVIRUS STRAIN
摘要
Abstract
A virus strain designated NM575 was isolated from a nasal swab of cattle with respiratory disease from a cattle farm in Inner Mongolia and analyzed in this study. The results showed that the virus could replicate in MDBK cell cultures containing 100 μg/mL DNA inhibitor (5-bromo-2-deoxyuridine, BRDU) and was identified as RNA type. Nonenveloped spherical virions were observed under electron microscope with a diameter of 30 nm, and in agreement with the characteristics of picornaviruses. Physiochemical analysis showed that the isolated virus had certain resistance to ether, chloroform, trypsin and acid. The high temperature could destroy the virus and even inactivate the virus, but 1 mol/L MgSO4could significantly improve the virus resistance to heat. These physiochemical properties were in agreement with those of enteroviruses. A fragment could be amplified with the specific primers for P1 gene of bovine enterovirus with RT-PCR. Then the amplified fragment was cloned and sequenced, and its nucleotide sequence was blasted in GenBank. The blast results showed that the nucleotide sequence of NM575 P1 gene had a 80% homology with that of enterovirus strain BEV-261. The phylogenetic tree indicated that the NM575 was an enterovirus and typed as EV-F1. The results of growth characteristics of NM575 in cell cultures showed that the virus could replicate in cell cultures originated from cattle, hamster, pig, dog and chicken, and especially in MDBK cell culture with a titer of 107.6TCID50/mL. In conclusion, the results presented in this study demonstrated that the NM575 strain was an enterovirus. So far, this was the first report for isolating bovine enterovirus from the nasal swab of cattle in China.关键词
牛肠道病毒/分离/鉴定/理化特性Key words
Bovine enterovirus/isolation/identification/physicochemical properties分类
农业科技引用本文复制引用
宋文凤,向文杰,林俊,李德栋,陈瑞红,朱远茂,薛飞..一株牛肠道病毒的分离与鉴定[J].中国动物传染病学报,2018,26(3):34-39,6.基金项目
国家自然科学基金(31372452) （31372452）
国家重点研发计划(2016YFD0500908) （2016YFD0500908）
