中国动物传染病学报2018，Vol.26Issue(3)：40-44,5.
低含量血清培养IBRS-2细胞及其增殖猪细小病毒N株的研究
STUDY ON CULTURE OF IBRS-2 CELLS WITH LOW SERUM CONCENTRATION AND PROPAGATION OF PORCINE PARVOVIRUS STRAIN N
摘要
Abstract
In this study, the MEM containing 8%, 4%, 2% and 1% sera, respectively, were used to culture IBRS-2 cells and to proliferate Porcine parvovirus(PPV) N strain. The results showed that IBRS-2 cells were in good growth state in concentrations of 8%, 4% and 2% sera culture medium, respectively, but when the concentration of the serum was decreased to 1%, the cells were in poor attachment and growth. Meanwhile, the densities of IBRS-2 cells at 0, 24, 48 ,72 and 96 h time point in concentrations of 8%, 4% and 2% serum culture medium was not significantly different with the control. Thereby, the culture media containing 2% serum were selected for cell cultivation and virus yield, and the virus titers were determined by TCID50and hemagglutination test. The results showed that the PPV N strain average titer of PPV N strain in 2% serum was not significantly different from that in routine culture (6%～10% serum) , indicating that the system could be used for the proliferation of PPV N strain. Results show that this study successfully domesticated out the IBRS-2 cells that could grow well under low serum culture conditions (containing 2% serum), and the PPV N strains could proliferate well in the lower serum cultured IBRS - 2 cells.关键词
IBRS-2细胞/低含量血清/猪细小病毒Key words
IBRS-2 cells/low serum/PPV N strain分类
农业科技引用本文复制引用
卢冰霞,卢敬专,李斌,苏乾莲,陈忠伟,段群棚,梁家幸,周英宁,赵武,何颖,闭炳芬,秦毅斌,蒋冬福..低含量血清培养IBRS-2细胞及其增殖猪细小病毒N株的研究[J].中国动物传染病学报,2018,26(3):40-44,5.基金项目
广西公益性科研院所基本科研业务费专项项目(桂科专项15-2、16-2) （桂科专项15-2、16-2）
广西水产畜牧科技项目(桂渔牧科201528030、201633034). （桂渔牧科201528030、201633034）
