中国动物传染病学报2018，Vol.26Issue(3)：45-50,6.
血清2型鸭疫里默氏杆菌铁载体受体蛋白突变体的构建及生物学特性研究
THE MUTANT CONSTRUCTION OF THE SIDEROPHORE RECEPTOR PROTEIN AND BIOLOGICAL CHARACTERISTICS STUDY IN RIEMERELLA ANATIPESTIFER SEROTYPE 2
摘要
Abstract
Iron is an important growth factor of pathogenic bacterium, the research shows that the iron uptake system regulates Riemerella anatipestifer iron metabolism and is one of the important pathogenic factor of Riemerella anatipestifer. In this study, the relationship between Riemerella anatipestifer ATP dependent outer membrane siderophore receptor protein(SRP) and iron metabolism, bacterial virulence factor was investigated. The mutant of SRP protein was constructed and the growth curve test showed that the growth of the mutant strain was slower about 10 hours than that of wild type strain in the iron limited environment, it indicated that SRP protein was one of the essential factors of Riemerella anatipestifer infection and had a vital role in maintaining Riemerella anatipestifer iron metabolism in vivo . The animal experiment showed that the dead time of duck infected the mutant was later 24 hours than that of the wild type strain. It showed that the growth rate of mutant strain and the speed reaching lethal number was obviously later than the wild type. Thus it indicated that SRP was one of the important virulence factor in Riemerella anatipestifer.关键词
鸭疫里默氏杆菌/铁离子摄取系统/铁载体受体蛋白/毒力因子Key words
Riemerella anatipestifer/iron uptake system/siderophore receptor protein/virulence factor分类
农业科技引用本文复制引用
董嘉文,孙敏华,李林林,马海彬,龚凤平,罗梦萍,王贵平,袁建丰..血清2型鸭疫里默氏杆菌铁载体受体蛋白突变体的构建及生物学特性研究[J].中国动物传染病学报,2018,26(3):45-50,6.基金项目
广州市科技计划项目(201510010250,201704020083) （201510010250,201704020083）
国家重点研发计划(2017YFD0500800) （2017YFD0500800）
广东省科技计划项目(2014A020208052,2015B020230004,2015B070701015) （2014A020208052,2015B020230004,2015B070701015）
广东省科技发展专项资金项目(2016B020234006, 2017B090904029) （2016B020234006, 2017B090904029）
广东省技术开发及产业化类别(2015B020203006) （2015B020203006）
广州市科技发展专项资金项目重点项目专题(201804020006) （201804020006）
广东省新型研发机构建设——初创期补贴专题:广东省现代农业产业技术体系创新团队(2017LM2150) （2017LM2150）
